Functional expression cloning reveals proapoptotic role for protein phosphatase 4

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dc.contributor.authorMourtada-Maarabouni, M.
dc.contributor.authorKirkham, L.
dc.contributor.authorJenkins, B.
dc.contributor.authorRayner, J.
dc.contributor.authorGonda, T.
dc.contributor.authorStarr, R.
dc.contributor.authorTrayner, I.
dc.contributor.authorFarzaneh, F.
dc.contributor.authorWilliams, G.
dc.date.issued2003
dc.description© 2003 Nature Publishing Group All rights reserved
dc.description.abstractFunctional expression cloning strategies are highly suitable for the analysis of the molecular control of apoptosis. This approach has two critical advantages. Firstly, it eliminates prior assumptions about the properties of the proteins involved, and, secondly, it selectively targets proteins that are causally involved in apoptosis control and which affect the crucial cellular decision between survival and death. The application of this strategy to the isolation of cDNAs conferring resistance to dexamethasone and italic gamma-irradiation resulted in the isolation of a partial cDNA for the catalytic subunit of protein phosphatase 4 (PP4). Cells transfected with this partial cDNA in an expression vector downregulated PP4 and were resistant to both dexamethasone and UV radiation, as demonstrated by both membrane integrity and colony-forming assays. These observations suggest that PP4 plays an important proapoptotic role in T lymphocytes.
dc.description.statementofresponsibilityM. Mourtada-Maarabouni, L. Kirkham, B. Jenkins, J. Rayner, T.J. Gonda, R. Starr, I. Trayner, F. Farzaneh and G.T. Williams
dc.identifier.citationCell Death and Differentiation, 2003; 10(9):1016-1024
dc.identifier.doi10.1038/sj.cdd.4401274
dc.identifier.issn1350-9047
dc.identifier.issn1476-5403
dc.identifier.orcidJenkins, B. [0000-0002-7552-4656]
dc.identifier.orcidGonda, T. [0000-0002-8792-3021]
dc.identifier.urihttp://hdl.handle.net/2440/56123
dc.language.isoen
dc.publisherNature Publishing Group
dc.source.urihttps://doi.org/10.1038/sj.cdd.4401274
dc.subjectPP4
dc.subjectdephosphorylation
dc.subjectphosphatase
dc.subjectapoptosis
dc.titleFunctional expression cloning reveals proapoptotic role for protein phosphatase 4
dc.typeJournal article
pubs.publication-statusPublished

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