Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates

Date

2008

Authors

Brotherton, P.
Endicott, P.
Beaumont, M.
Barnett, R.
Austin, J.
Cooper, A.
Sanchez, J.

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Journal article

Citation

Forensic Science International: Genetics Supplement Series, 2008; 1(1):19-21

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Paul Brotherton, Phillip Endicott, Mark Beaumont, Ross Barnett, Jeremy Austin, Alan Cooper, Juan J. Sanchez

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DOI

10.1016/j.fsigss.2007.10.111

Abstract

High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus. © 2008 Elsevier Ireland Ltd. All rights reserved.

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https://doi.org/10.1016/j.fsigss.2007.10.111

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http://hdl.handle.net/2440/51618