Structure and chromosomal localization of the genomic locus encoding the Kiz1 LIM-Kinase gene

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dc.contributor.authorBernard, O.
dc.contributor.authorBurkitt, V.
dc.contributor.authorWebb, G.
dc.contributor.authorBottema, C.
dc.contributor.authorNicholl, J.
dc.contributor.authorSutherland, G.
dc.contributor.authorMatthew, P.
dc.date.issued1996
dc.description.abstractWe have cloned and characterized the mouse gene encoding Kiz1/Limk1, a new member of the zinc-finger LIM family that also has a kinase domain. The gene encompasses 25 kb of the mouse genome, and the organization of its 16 exons does not correlate with its functional domains. The promoter region of Kiz1/Limk1 was identified by cloning a 1.06-kb genomic fragment upstream from the first ATG in a promotorless CAT vector. This construct was demonstrated to drive CAT expression in Jurkat cells. The promoter sequence lacks conventional TATA and CAAT motifs but contains consensus binding sequences for several transcriptional regulators implicated in control of transcription in many different cell types, including Sp1, Ets, and E2A. Analysis of the chromosomal localization of KIZ1/LIMK1 indicates that it lies on human chromosome 17 in the region 17q25 and on mouse Chromosome 5, band G2.
dc.description.statementofresponsibilityBernard, Ora ; Burkitt, Vicki ; Webb, Graham C. ; Bottema, Cynthia D.K. ; Nicholl, Jillian ; Sutherland, Grant R. ; Matthew, Paul
dc.identifier.citationGenomics, 1996; 35(3):593-596
dc.identifier.doi10.1006/geno.1996.0403
dc.identifier.issn0888-7543
dc.identifier.issn1089-8646
dc.identifier.orcidBottema, C. [0000-0001-6245-0099]
dc.identifier.urihttp://hdl.handle.net/2440/13235
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.source.urihttps://doi.org/10.1006/geno.1996.0403
dc.subjectJurkat Cells
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectProtein Kinases
dc.subjectDNA-Binding Proteins
dc.subjectDNA, Complementary
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectChromosome Mapping
dc.subjectBase Sequence
dc.subjectZinc Fingers
dc.subjectExons
dc.subjectMolecular Sequence Data
dc.subjectLim Kinases
dc.subjectPromoter Regions, Genetic
dc.subjectProtein Serine-Threonine Kinases
dc.titleStructure and chromosomal localization of the genomic locus encoding the Kiz1 LIM-Kinase gene
dc.typeJournal article
pubs.publication-statusPublished

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