Myb-binding protein 1A (MYBBP1A) is essential for early embryonic development, controls cell cycle and mitosis, and acts as a tumor suppressor

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dc.contributor.authorMori, S.
dc.contributor.authorBernardi, R.
dc.contributor.authorLaurent, A.
dc.contributor.authorResnati, M.
dc.contributor.authorCrippa, A.
dc.contributor.authorGabrieli, A.
dc.contributor.authorKeough, R.
dc.contributor.authorGonda, T.
dc.contributor.authorBlasi, F.
dc.contributor.editorPlow, E.
dc.date.issued2012
dc.description.abstractMYBBP1A is a predominantly nucleolar transcriptional regulator involved in rDNA synthesis and p53 activation via acetylation. However little further information is available as to its function. Here we report that MYBBP1A is developmentally essential in the mouse prior to blastocyst formation. In cell culture, down-regulation of MYBBP1A decreases the growth rate of wild type mouse embryonic stem cells, mouse embryo fibroblasts (MEFs) and of human HeLa cells, where it also promotes apoptosis. HeLa cells either arrest at G2/M or undergo delayed and anomalous mitosis. At mitosis, MYBBP1A is localized to a parachromosomal region and gene-expression profiling shows that its down-regulation affects genes controlling chromosomal segregation and cell cycle. However, MYBBP1A down-regulation increases the growth rate of the immortalized NIH3T3 cells. Such Mybbp1a down-regulated NIH3T3 cells are more susceptible to Ras-induced transformation and cause more potent Ras-driven tumors. We conclude that MYBBP1A is an essential gene with novel roles at the pre-mitotic level and potential tumor suppressor activity.
dc.description.statementofresponsibilitySilvia Mori, Rosa Bernardi, Audrey Laurent, Massimo Resnati, Ambra Crippa, Arianna Gabrieli, Rebecca Keough, Thomas J. Gonda, Francesco Blasi
dc.identifier.citationPLoS ONE, 2012; 7(10):e39723-1-e39723-14
dc.identifier.doi10.1371/journal.pone.0039723
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.orcidGonda, T. [0000-0002-8792-3021]
dc.identifier.urihttp://hdl.handle.net/2440/94805
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights© Mori et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1371/journal.pone.0039723
dc.subjectCells, Cultured
dc.subjectHela Cells
dc.subjectNIH 3T3 Cells
dc.subjectFibroblasts
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectMice, Knockout
dc.subjectHumans
dc.subjectMice
dc.subjectMice, Nude
dc.subjectCell Transformation, Neoplastic
dc.subjectCarrier Proteins
dc.subjectNucleocytoplasmic Transport Proteins
dc.subjectRNA-Binding Proteins
dc.subjectDNA-Binding Proteins
dc.subjectTumor Suppressor Proteins
dc.subjectNuclear Proteins
dc.subjectTranscription Factors
dc.subjectCell Cycle
dc.subjectMitosis
dc.subjectApoptosis
dc.subjectCell Proliferation
dc.subjectGene Expression Regulation, Developmental
dc.subjectRNA Interference
dc.subjectPregnancy
dc.subjectFemale
dc.subjectMale
dc.subjectEmbryonic Stem Cells
dc.subjectEmbryo, Mammalian
dc.subjectHEK293 Cells
dc.titleMyb-binding protein 1A (MYBBP1A) is essential for early embryonic development, controls cell cycle and mitosis, and acts as a tumor suppressor
dc.typeJournal article
pubs.publication-statusPublished

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