Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition

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dc.contributor.authorHyles, J.
dc.contributor.authorVautrin, S.
dc.contributor.authorPettolino, F.
dc.contributor.authorMacMillan, C.
dc.contributor.authorStachurski, Z.
dc.contributor.authorBreen, J.
dc.contributor.authorBerges, H.
dc.contributor.authorWicker, T.
dc.contributor.authorSpielmeyer, W.
dc.date.issued2017
dc.description.abstractThe tiller inhibition gene (tin) that reduces tillering in wheat (Triticum aestivum) is also associated with large spikes, increased grain weight, and thick leaves and stems. In this study, comparison of near-isogenic lines (NILs) revealed changes in stem morphology, cell wall composition, and stem strength. Microscopic analysis of stem cross-sections and chemical analysis of stem tissue indicated that cell walls in tin lines were thicker and more lignified than in free-tillering NILs. Increased lignification was associated with stronger stems in tin plants. A candidate gene for tin was identified through map-based cloning and was predicted to encode a cellulose synthase-like (Csl) protein with homology to members of the CslA clade. Dinucleotide repeat-length polymorphism in the 5′UTR region of the Csl gene was associated with tiller number in diverse wheat germplasm and linked to expression differences of Csl transcripts between NILs. We propose that regulation of Csl transcript and/or protein levels affects carbon partitioning throughout the plant, which plays a key role in the tin phenotype.
dc.description.statementofresponsibilityJ. Hyles, S. Vautrin, F. Pettolino, C. MacMillan, Z. Stachurski, J. Breen, H. Berges, T. Wicker, and W. Spielmeyer
dc.identifier.citationJournal of Experimental Botany, 2017; 68(7):1519-1529
dc.identifier.doi10.1093/jxb/erx051
dc.identifier.issn0022-0957
dc.identifier.issn1460-2431
dc.identifier.orcidBreen, J. [0000-0001-6184-0925]
dc.identifier.urihttp://hdl.handle.net/2440/105577
dc.language.isoen
dc.publisherOxford University Press
dc.rights© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1093/jxb/erx051
dc.subjectCellulose synthase-like; cell wall thickening; lignin; lodging; tillering; tin; Triticum aestivum; wheat
dc.titleRepeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition
dc.typeJournal article
pubs.publication-statusPublished

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