The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering

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dc.contributor.authorStreck, S.
dc.contributor.authorClulow, A.J.
dc.contributor.authorNielsen, H.M.
dc.contributor.authorRades, T.
dc.contributor.authorBoyd, B.J.
dc.contributor.authorMcDowell, A.
dc.date.issued2019
dc.description.abstractHypothesis: The distribution of three cell-penetrating peptides (CPPs) with different architectures (short, long linear and branched) on poly(lactic-co-glycolic) acid (PLGA) nanoparticles depends on the conjugation approach. Here, we explore the utilization of a zero-length crosslinking reaction for the covalent attachment of CPPs to PLGA nanoparticles and the translation of the reaction into a microfluidic platform. Experiments: A microfluidic device with a staggered herringbone mixer was used for the formulation of CPP-tagged PLGA nanoparticles. CPP-tagged PLGA nanoparticles were labeled with gold nanoparticles (AuNPs) and transmission electron microscopy (TEM) and small angle X-ray scattering (SAXS) were used to elucidate the distribution of CPPs. Findings: The SAXS scattering profiles for the CPP-tagged PLGA nanoparticles prepared with the in situ microfluidics conjugation approach indicated a distribution of the Au-labeled CPPs throughout the PLGA nanoparticles. For the post-microfluidics conjugation approach, the SAXS scattering profiles did not show the feature of the Au-labeled CPPs distributed throughout the PLGA nanoparticles and an arrangement of the Au-labeled CPP on the surface was support by TEM micrographs. The distribution of the CPPs was highly dependent on the conjugation approach and was not influenced by the architecture of the CPPs. The results provided insight for the rational design of CPP-tagged PLGA nanoparticles using microfluidics.
dc.description.statementofresponsibilitySarah Streck, Andrew J. Clulow, Hanne Mørck Nielsen, Thomas Rades, Ben J. Boyd, Arlene McDowell
dc.identifier.citationJournal of Colloid and Interface Science, 2019; 555:438-448
dc.identifier.doi10.1016/j.jcis.2019.08.007
dc.identifier.issn0021-9797
dc.identifier.issn1095-7103
dc.identifier.orcidRades, T. [0000-0002-7521-6020]
dc.identifier.urihttps://hdl.handle.net/2440/133519
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/arc/DP160102906
dc.relation.granthttp://purl.org/au-research/grants/arc/DE190100531
dc.rights© 2019 Elsevier Inc. All rights reserved
dc.source.urihttps://doi.org/10.1016/j.jcis.2019.08.007
dc.subjectPLGA nanoparticles; TAT; Branched cell-penetrating peptide; Microfluidics; Small angle X-ray scattering; Transmission electron microscopy
dc.subject.meshHumans
dc.subject.meshX-Ray Diffraction
dc.subject.meshMicrofluidic Analytical Techniques
dc.subject.meshParticle Size
dc.subject.meshSurface Properties
dc.subject.meshNanoparticles
dc.subject.meshScattering, Small Angle
dc.subject.meshCell-Penetrating Peptides
dc.subject.meshPolylactic Acid-Polyglycolic Acid Copolymer
dc.titleThe distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering
dc.typeJournal article
pubs.publication-statusPublished

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