miR-142-5p in bone marrow-derived Mesenchymal stem cells promotes osteoporosis involving targeting adhesion molecule VCAM-1 and inhibiting cell migration

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dc.contributor.authorTeng, Z.
dc.contributor.authorXie, X.
dc.contributor.authorZhu, Y.
dc.contributor.authorLiu, J.
dc.contributor.authorHu, X.
dc.contributor.authorNa, Q.
dc.contributor.authorZhang, X.
dc.contributor.authorWei, G.
dc.contributor.authorXu, S.
dc.contributor.authorLiu, Y.
dc.contributor.authorZhang, X.
dc.contributor.authorXian, C.J.
dc.date.issued2018
dc.description.abstractOsteoporosis is a systemic bone metabolic disease that is highly prevalent in the elderly population, particularly in postmenopausal women, which results in enhanced bone fragility and an increased susceptibility to fractures. However, the underlying molecular pathogenesis mechanisms still remain to be further elucidated. In this study, in a rat ovariectomy- (OVX-) induced postmenopausal osteoporosis model, aberrant expression of a microRNA miR-142-5p and vascular cell adhesion molecule 1 (VCAM-1) was found by RNA sequencing analysis and qRT-PCR. Using a dual-luciferase reporter assay, we found that miR-142-5p can bind to and decrease expression of VCAM-1 mRNA. Such reduction was prohibited when the miR-142-5p binding site in VCAM-1 3'UTR was deleted, and Western blotting analyses validated the fact that miR-142-5p inhibited the expression of VCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. Collectively, these data indicated the important role of miR-142-5p in osteoporosis development involving targeting VCAM-1 and inhibiting BMMSC migration.
dc.description.statementofresponsibilityZhaowei Teng, Xueguan Xie, Yun Zhu, Jianping Liu, Xingbo Hu, Qiang Na, Xiongwen Zhang, Guojun Wei, Shen Xu, Yugang Liu, Xiguang Zhang and Cory J. Xian
dc.identifier.citationBioMed Research International, 2018; 2018:3274641-1-3274641-7
dc.identifier.doi10.1155/2018/3274641
dc.identifier.issn2314-6133
dc.identifier.issn2314-6141
dc.identifier.orcidXian, C.J. [0000-0002-8467-2845]
dc.identifier.urihttp://hdl.handle.net/2440/114132
dc.language.isoen
dc.publisherHindawi
dc.relation.grant81660156
dc.relation.grant81760136
dc.relation.grant81671928
dc.relation.grant2014FZ048
dc.relation.grant2017FE468-181
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1042105
dc.rights© 2018 Zhaowei Teng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1155/2018/3274641
dc.subjectBone Marrow Cells
dc.subjectMesenchymal Stem Cells
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectOsteoporosis
dc.subjectVascular Cell Adhesion Molecule-1
dc.subjectMicroRNAs
dc.subject3' Untranslated Regions
dc.subjectCell Movement
dc.subjectGene Expression Regulation
dc.subjectFemale
dc.titlemiR-142-5p in bone marrow-derived Mesenchymal stem cells promotes osteoporosis involving targeting adhesion molecule VCAM-1 and inhibiting cell migration
dc.typeJournal article
pubs.publication-statusPublished

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