New phenolic grape skin products from vitis vinifera cv. Pinot noir
Date
2011
Authors
Kneknopoulos, P.
Skouroumounis, G.
Hayasaka, Y.
Taylor, D.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Journal of Agricultural and Food Chemistry, 2011; 59(3):1005-1011
Statement of Responsibility
Petros Kneknopoulos, George K. Skouroumounis, Yoji Hayasaka, and Dennis K. Taylor
Conference Name
Abstract
Anthocyanins and their related compounds were extracted from grape skins of Pinot noir, using 50% aqueous methanol, and purified by solid phase extraction chromatography using XAD-7 resin to obtain a pigment-rich fraction. This fraction was subjected to multilayer coil countercurrent chromatography (MLCCC) using a quaternary solvent system consisting of tert-butyl methyl ether/n-butanol/acetonitrile/water acidified with 0.01% trifluoroacetic acid (2:2:0.1-1.8:5) (v/v/v/v) in a step gradient elution to separate anthocyanin oligomers from grape anthocyanins. In the process of the characterization of the MLCCC fractions by electrospray mass spectrometry, two noncolored anthocyanin derivatives were found and characterized on the basis of their mass spectral data. As a result, these compounds have been tentatively identified as coupling products between both hydrated malvidin-3-glucoside and peonidin-3-glucoside, with 2-S-glutathionyl caffeoyl tartaric acid (GRP). It is therefore proposed that grape skins contain this new class of coupling product, and a possible chemical pathway for their formation is suggested.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
©2011 American Chemical Society