Mutations of the ca²⁺-sensing stromal interaction molecule stim1 regulate ca²⁺ influx by altered oligomerization of stim1 and by destabilization of the ca²⁺ channel orai1

Simple item page
dc.contributor.authorKilch, T.
dc.contributor.authorAlansary, D.
dc.contributor.authorPeglow, M.
dc.contributor.authorDorr, K.
dc.contributor.authorRychkov, G.
dc.contributor.authorRieger, H.
dc.contributor.authorPeinelt, C.
dc.contributor.authorNiemeyer, B.
dc.date.issued2013
dc.description.abstractA drop of endoplasmic reticulum Ca²⁺ concentration triggers its Ca²⁺ ssensor protein stromal interaction molecule 1 (STIM1) to oligomerize and accumulate within endoplasmic reticulum-plasma membrane junctions where it activates Orai1 channels, providing store-operated Ca²⁺ entry. To elucidate the functional significance of N-glycosylation sites of STIM1, we created different mutations of asparagine-131 and asparagine-171. STIM1 NN/DQ resulted in a strong gain of function. Patch clamp, Total Internal Reflection Fluorescent (TIRF) microscopy, and fluorescence recovery after photobleaching (FRAP) analyses revealed that expression of STIM1 DQ mutants increases the number of active Orai1 channels and the rate of STIM1 translocation to endoplasmic reticulum-plasma membrane junctions with a decrease in current latency. Surprisingly, co-expression of STIM1 DQ decreased Orai1 protein, altering the STIM1:Orai1 stoichiometry. We describe a novel mathematical tool to delineate the effects of altered STIM1 or Orai1 diffusion parameters from stoichiometrical changes. The mutant uncovers a novel mechanism whereby “superactive” STIM1 DQ leads to altered oligomerization rate constants and to degradation of Orai1 with a change in stoichiometry of activator (STIM1) to effector (Orai1) ratio leading to altered Ca²⁺ homeostasis.
dc.description.statementofresponsibilityTatiana Kilch, Dalia Alansary, Martin Peglow, Kathrin Dörr, Grigori Rychkov, Heiko Rieger, Christine Peinelt, and Barbara A. Niemeyer
dc.identifier.citationJournal of Biological Chemistry, 2013; 288(3):1653-1664
dc.identifier.doi10.1074/jbc.M112.417246
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.orcidRychkov, G. [0000-0002-2788-2977]
dc.identifier.urihttp://hdl.handle.net/2440/78371
dc.language.isoen
dc.publisherAmer Soc Biochemistry Molecular Biology Inc
dc.rights© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.source.urihttps://doi.org/10.1074/jbc.m112.417246
dc.subjectCalcium Channels
dc.subjectCalcium Imaging
dc.subjectCalcium Signaling
dc.subjectGlycosylation
dc.subjectProtein Degradation
dc.subjectMonte Carlo Simulation
dc.subjectOrai1
dc.subjectSTIM1
dc.subjectTIRF
dc.subjectDiffusion Trap
dc.titleMutations of the ca²⁺-sensing stromal interaction molecule stim1 regulate ca²⁺ influx by altered oligomerization of stim1 and by destabilization of the ca²⁺ channel orai1
dc.title.alternativeMutations of the ca(2+)-sensing stromal interaction molecule stim1 regulate ca(2+) influx by altered oligomerization of stim1 and by destabilization of the ca(2+) channel orai1
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Physiology publications