A combined 13CO2/H2 breath test can be used to assess starch digestion and fermentation in humans
| dc.contributor.author | Symonds, E. | |
| dc.contributor.author | Kritas, S. | |
| dc.contributor.author | Omari, T. | |
| dc.contributor.author | Butler, R. | |
| dc.date.issued | 2004 | |
| dc.description.abstract | Ingestion of starch from corn (naturally enriched with (13)C) should produce (13)CO(2) after small intestinal digestion and (13)CO(2) and H(2) from colonic fermentation. This study used a combined (13)CO(2)/H(2) breath test to assess the digestion and fermentation of resistant starch and to show that the test could detect changes in digestibility due to cooking. Volunteers consumed 40 g digestible cornstarch with water (n = 8), or 40 g resistant cornstarch in liquid (n = 12) or cooked into a pancake (n = 4). Interval breath sampling was performed and analyzed for (13)CO(2) and H(2). Ingestion of resistant starch produced a double-peaked (13)CO(2) excretion curve. The first increase in (13)CO(2) occurred at the same time as excretion from digestible starch (55 +/- 9 and 68 +/- 9 min, respectively), which was due to small intestinal digestion. The second increase in (13)CO(2) was accompanied by an increase in H(2) excretion (432 +/- 15 and 428 +/- 48 min, respectively), which was indicative of colonic bacterial fermentation. Cooking resistant starch increased its degree of digestion from 36 to 72%. The (13)CO(2)/H(2) breath test can be used to estimate digestion and fermentation of starches in different physiologic and pathologic conditions. | |
| dc.identifier.citation | Journal of Nutrition, 2004; 134(5):1193-1196 | |
| dc.identifier.doi | 10.1093/jn/134.5.1193 | |
| dc.identifier.issn | 0022-3166 | |
| dc.identifier.issn | 1541-6100 | |
| dc.identifier.orcid | Omari, T. [0000-0001-5108-7378] | |
| dc.identifier.uri | http://hdl.handle.net/2440/7545 | |
| dc.language.iso | en | |
| dc.publisher | Amer Inst Nutrition | |
| dc.source.uri | https://doi.org/10.1093/jn/134.5.1193 | |
| dc.subject | Humans | |
| dc.subject | Carbon Dioxide | |
| dc.subject | Hydrogen | |
| dc.subject | Carbon Isotopes | |
| dc.subject | Starch | |
| dc.subject | Breath Tests | |
| dc.subject | Fermentation | |
| dc.subject | Digestion | |
| dc.subject | Adult | |
| dc.subject | Female | |
| dc.subject | Cooking | |
| dc.title | A combined 13CO2/H2 breath test can be used to assess starch digestion and fermentation in humans | |
| dc.type | Journal article | |
| pubs.publication-status | Published |