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Kinetic optimisation of open-tubular liquid-chromatography capillaries coated with thick porous layers for increased loadability

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dc.contributor.authorCauson, T.J.
dc.contributor.authorShellie, R.A.
dc.contributor.authorHilder, E.F.
dc.contributor.authorDesmet, G.
dc.contributor.authorEeltink, S.
dc.date.issued2011
dc.description.abstractThe kinetic optimisation of open-tubular liquid chromatography (OTLC) columns has been revisited by taking the thick-film effects for porous coatings on retention, column resistance, band broadening and mass loadability into account. Considering the most advantageous case (i.e. where the retentive layer allows for the same high internal diffusion coefficient as conventional porous particles), calculations show the need for the development of coating procedures leading to porous films filling up approximately 50-70% of the total column diameter. Furthermore, to achieve optimum kinetic performance for separations of small molecules with total analysis times of less than 8. h (k'=9), total column diameters should be less than 6μm with lengths typically greater than 0.8. m for N values of 125,000-500,000 at a pressure of 400. bar. The use of elevated temperature LC (90°C) is also shown to increase the allowable total column diameter to up to 9μm for a larger range of N values (100,000-880,000).
dc.identifier.citationJournal of Chromatography A, 2011; 1218(46):8388-8393
dc.identifier.doi10.1016/j.chroma.2011.09.047
dc.identifier.issn0021-9673
dc.identifier.issn1873-3778
dc.identifier.urihttps://hdl.handle.net/11541.2/116206
dc.language.isoen
dc.publisherElsevier
dc.relation.fundingARC DP0987318
dc.relation.fundingARC FT0990521
dc.relation.fundingARC DP110104923
dc.relation.fundingResearch Foundation Flanders G.0919.09
dc.rightsCopyright 2011 Elsevier B.V.
dc.source.urihttps://doi.org/10.1016/j.chroma.2011.09.047
dc.subjectcoating
dc.subjectkinetic plots
dc.subjectPLOT columns
dc.titleKinetic optimisation of open-tubular liquid-chromatography capillaries coated with thick porous layers for increased loadability
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915979810601831

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