Fast X-ray fluorescence microscopy provides high-throughput phenotyping of element distribution in seeds
Date
2023
Authors
Ren, Z.W.
Yang, M.
McKenna, B.A.
Lian, X.M.
Zhao, F.J.
Kopittke, P.M.
Lombi, E.
Wang, P.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Plant Physiology, 2023; 191(3):1520-1534
Statement of Responsibility
Conference Name
Abstract
The concentration, chemical speciation, and spatial distribution of essential and toxic mineral elements in cereal seeds have important implications for human health. To identify genes responsible for element uptake, translocation, and storage, high-throughput phenotyping methods are needed to visualize element distribution and concentration in seeds. Here, we used X-ray fluorescence microscopy (mu-XRF) as a method for rapid and high-throughput phenotyping of seed libraries and developed an ImageJ-based pipeline to analyze the spatial distribution of elements. Using this method, we nondestructively scanned 4,190 ethyl methanesulfonate (EMS)-mutagenized M1 rice (Oryza sativa) seeds and 533 diverse rice accessions in a genome-wide association study (GWAS) panel to simultaneously measure concentrations and spatial distribution of elements in the embryo, endosperm, and aleurone layer. A total of 692 putative mutants and 65 loci associated with the spatial distribution of elements in rice seed were identified. This powerful method provides a basis for investigating the genetics and molecular mechanisms controlling the accumulation and spatial variations of mineral elements in plant seeds.Fast X-ray fluorescence microscopy (mu-XRF) provides a high-throughput method to investigate the mechanisms controlling the accumulation and spatial variations of mineral elements in plant seeds.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright 2022 American Society of Plant Biologists