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Real-time histology in liver disease usingmultiphoton microscopy with fluorescencelifetime imaging

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dc.contributor.authorWang, H.
dc.contributor.authorLiang, X.
dc.contributor.authorMohammed, Y.H.
dc.contributor.authorThomas, J.A.
dc.contributor.authorBridle, K.R.
dc.contributor.authorThorling, C.A.
dc.contributor.authorGrice, J.E.
dc.contributor.authorXu, Z.P.
dc.contributor.authorLiu, X.
dc.contributor.authorCrawford, D.H.G.
dc.contributor.authorRoberts, M.S.
dc.date.issued2015
dc.description.abstractConventional histology with light microscopy is essential in the diagnosis of most liver diseases. Recently, a concept of real-time histology with optical biopsy has been advocated. In this study, live mice livers (normal, with fibrosis, steatosis, hepatocellular carcinoma and ischemia-reperfusion injury) were imaged by MPM-FLIM for stain-free real-time histology. The acquired MPM-FLIM images were compared with conventional histological images. MPM-FLIM imaged subsurface cellular and subcellular histopathological hallmarks of live liver in mice models at high resolution. Additional information such as distribution of stellate cell associated auto fluorescence and fluorescence lifetime changes was also gathered by MPM-FLIM simultaneously, which cannot be obtained from conventional histology. MPM-FLIM could simultaneously image and quantify the cellular morphology and microenvironment of live livers without conventional biopsy or fluorescent dyes. We anticipate that in the near future MPM-FLIM will be evaluated from bench to bedside, leading to real-time histology and dynamic monitoring of human liver diseases.
dc.identifier.citationBiomedical Optics Express, 2015; 9(3):780-792
dc.identifier.doi10.1364/BOE.6.000780
dc.identifier.issn2156-7085
dc.identifier.issn2156-7085
dc.identifier.urihttps://hdl.handle.net/11541.2/114930
dc.language.isoen
dc.publisherOptical Society of America
dc.relation.fundingNational Health and Medical Research Council APP1049979
dc.relation.fundingAustralian Research Council ARC120104792
dc.rightsCopyright 2015 Optical Society of America
dc.source.urihttps://doi.org/10.1364/BOE.6.000780
dc.subjectliver disease
dc.subjectfluorescent dyes
dc.subjectdynamic monitoring
dc.subjectliver
dc.titleReal-time histology in liver disease usingmultiphoton microscopy with fluorescencelifetime imaging
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915928611301831

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