Contribution of serotype and genetic background to virulence of serotype 3 and serogroup 11 pneumococcal isolates

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dc.contributor.authorMcAllister, L.
dc.contributor.authorOgunniyi, A.
dc.contributor.authorStroeher, U.
dc.contributor.authorLeach, A.
dc.contributor.authorPaton, J.
dc.contributor.editorCamilli, A.
dc.date.issued2011
dc.description.abstractThe capsular serotype has long been associated with the virulence of Streptococcus pneumoniae. Here we present an in-depth study of phenotypic and genetic differences between serotype 3 and serogroup 11 S. pneumoniae clinical isolates from both the general and indigenous populations of Australia. Both serotypes/groups included clonally unrelated strains with differences in well-known polymorphic virulence genes, such as nanA and pspA, as demonstrated by multilocus sequence typing and Western blot analysis. Nonetheless, the serotype 3 strains were consistently and significantly more virulent in mice than the serogroup 11 strains. Despite extensive genomic analysis, noncapsular genes common to one serotype/group but not the other were not identified. Nevertheless, following the conversion of a serotype 11A isolate to serotype 3 and subsequent analysis in an intranasal infection model, it was evident that both capsular and noncapsular factors determine the virulence phenotype in mice. However, it appears that these noncapsular factors vary from strain to strain.
dc.description.statementofresponsibilityLauren J. McAllister, Abiodun D. Ogunniyi, Uwe H. Stroeher, Amanda J. Leach, and James C. Paton
dc.identifier.citationInfection and Immunity, 2011; 79(12):4839-4849
dc.identifier.doi10.1128/IAI.05663-11
dc.identifier.issn0019-9567
dc.identifier.issn1098-5522
dc.identifier.orcidOgunniyi, A. [0000-0001-9308-5629]
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]
dc.identifier.urihttp://hdl.handle.net/2440/68720
dc.language.isoen
dc.publisherAmer Soc Microbiology
dc.rightsCopyright © 2011, American Society for Microbiology. All Rights Reserved.
dc.source.urihttps://doi.org/10.1128/iai.05663-11
dc.subjectAnimals
dc.subjectMice
dc.subjectStreptococcus pneumoniae
dc.subjectCellobiose
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectSerotyping
dc.subjectPolymerase Chain Reaction
dc.subjectVirulence
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenomic Islands
dc.subjectMutation
dc.subjectGenetic Variation
dc.titleContribution of serotype and genetic background to virulence of serotype 3 and serogroup 11 pneumococcal isolates
dc.typeJournal article
pubs.publication-statusPublished

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