Comparative Genomic Hybridization
Date
2006
Authors
Hussey, Nicole Dominique
Hu, Dong Gui
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Patent
Citation
Statement of Responsibility
Invented by N.D. Hussey, D.G. Hu; Assigned to Adelaide Research and Innovation Pty Ltd; Fan Zheng agent for the inventors.
Conference Name
Abstract
The present invention relates to a method of comparing at least one chromosome or part thereof from a cell with a first karyotype with the corresponding chromosome or part thereof from a cell with a second karyotype. The method includes the steps of: (a) amplifying DNA from an isolated chromosome or part of an isolated chromosome; (b) attaching the amplified DNA to a solid substrate; (c) amplifying DNA from one or more cells with a first karyotype and amplifying DNA from one or more cells with a second karyotype; (d) labelling the amplified DNA from the one or more cells with a first karyotype with a first label, and labelling the amplified DNA from the one or more cells with a second karyotype with a second label, wherein the first and second labels are detectably different; (e) hybridizing the amplified and labelled DNA from the one or more cells with a first karyotype to the amplified DNA attached to the solid substrate, and hybridizing the amplified and labelled DNA from the one or more cells with a second karyotype to the amplified DNA attached to the solid substrate; and (f) comparing the relative amount of first and second labels hybridized to the amplified DNA attached to the solid substrate.
School/Discipline
School of Paediatrics and Reproductive Health : Obstetrics and Gynaecology
Dissertation Note
Provenance
Description
PATENT: N.D. Hussey, D.G. Hu (Assigned to Adelaide Research and Innovation Pty Ltd). CN 200480015169 (PCT/AU04/00429) filed Apr. 2, 2004; published Jul. 5, 2006