Anti-ovine V CAM-1 monoclonal antibodies inhibit adhesion and proliferation between sheep endothelial and mononuclear cells in vitro.

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dc.contributor.authorGrooby, W.
dc.contributor.authorKrishnan, R.
dc.contributor.authorRuss, G.
dc.date.issued1997
dc.description.abstractThis paper reports the production and characterization of three monoclonal antibodies (mAb) recognizing ovine vascular cell adhesion molecule-1 (VCAM-1). The mAb were raised against sheep umbilical vein endothelial cells (ShUVEC) and flow cytometric analysis demonstrated that one mAb, QE4G9, was cross-reactive with human VCAM-1 expressed on Chinese hamster ovary cell transfectants. Protein modulation studies on ShUVEC and immunoperoxidase staining of inflamed renal tissue further indicated the reactivity of the other two ovine mAb, QE1F3 and QE2G4, with ovine VCAM-1. The flow cytometric profile of the three mAb on stimulated ShUVEC was identical to that observed with the cross-reactive anti-human VCAM- mAb, HAE2-1. Peak expression occurred between 6-12 h after stimulation, followed by a slight decrease to a plateau extending beyond 48 h. In functional assays, all mAb inhibited adhesion of PMA-activated sheep PBMC to stimulated ShUVEC. In addition, QE4G9 inhibited proliferation of sheep PBMC in the mixed lymphocyte-endothelial cell reaction (MLER) by 56%. The results demonstrate that the three anti-ovine VCAM-1 mAb recognize functional epitopes on sheep vascular endothelial cells. These mAb will be valuable tools in the investigation of VCAM-1 expression in various pathophysiological conditions using sheep models, and in the study of VCAM-1-mediated leucocyte-endothelial cell interactions, both in vitro and in vivo.
dc.identifier.citationImmunology and Cell Biology, 1997; 75(6):546-553
dc.identifier.doi10.1038/icb.1997.85
dc.identifier.issn0818-9641
dc.identifier.issn1440-1711
dc.identifier.urihttp://hdl.handle.net/2440/8884
dc.language.isoen
dc.publisherBLACKWELL SCIENCE
dc.source.urihttps://doi.org/10.1038/icb.1997.85
dc.subjectEndothelium, Vascular
dc.subjectLeukocytes, Mononuclear
dc.subjectCells, Cultured
dc.subjectTumor Cells, Cultured
dc.subjectAnimals
dc.subjectMice, Inbred BALB C
dc.subjectSheep
dc.subjectHumans
dc.subjectMice
dc.subjectVascular Cell Adhesion Molecule-1
dc.subjectAntibodies, Monoclonal
dc.subjectCell Adhesion
dc.subjectCell Division
dc.subjectAntibody Specificity
dc.subjectTissue Distribution
dc.subjectCricetinae
dc.titleAnti-ovine V CAM-1 monoclonal antibodies inhibit adhesion and proliferation between sheep endothelial and mononuclear cells in vitro.
dc.typeJournal article
pubs.publication-statusPublished

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