Immunomodulatory properties of stem cells from human exfoliated deciduous teeth

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dc.contributor.authorYamaza, T.
dc.contributor.authorKentaro, A.
dc.contributor.authorChen, C.
dc.contributor.authorLiu, Y.
dc.contributor.authorShi, Y.
dc.contributor.authorGronthos, S.
dc.contributor.authorWang, S.
dc.contributor.authorShi, S.
dc.date.issued2010
dc.descriptionExtent: 10p.
dc.description.abstractIntroduction: Stem cells from human exfoliated deciduous teeth (SHED) have been identified as a population of postnatal stem cells capable of differentiating into osteogenic and odontogenic cells, adipogenic cells, and neural cells. Herein we have characterized mesenchymal stem cell properties of SHED in comparison to human bone marrow mesenchymal stem cells (BMMSCs). Methods: We used in vitro stem cell analysis approaches, including flow cytometry, inductive differentiation, telomerase activity, and Western blot analysis to assess multipotent differentiation of SHED and in vivo implantation to assess tissue regeneration of SHED. In addition, we utilized systemic SHED transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. Results: We found that SHED are capable of differentiating into osteogenic and adipogenic cells, expressing mesenchymal surface molecules (STRO-1, CD146, SSEA4, CD73, CD105, and CD166), and activating multiple signaling pathways, including TGFβ, ERK, Akt, Wnt, and PDGF. Recently, BMMSCs were shown to possess an immunomodulatory function that leads to successful therapies for immune diseases. We examined the immunomodulatory properties of SHED in comparison to BMMSCs and found that SHED had significant effects on inhibiting T helper 17 (Th17) cells in vitro. Moreover, we found that SHED transplantation is capable of effectively reversing SLE-associated disorders in MRL/lpr mice. At the cellular level, SHED transplantation elevated the ratio of regulatory T cells (Tregs) via Th17 cells. Conclusions: These data suggest that SHED are an accessible and feasible mesenchymal stem cell source for treating immune disorders like SLE.
dc.description.statementofresponsibilityTakayoshi Yamaza, Akiyama Kentaro, Chider Chen, Yi Liu, Yufang Shi, Stan Gronthos, Songlin Wang, Songtao Shi
dc.identifier.citationCurrent Stem Cell Research and Therapy, 2010; 1(5):1-10
dc.identifier.doi10.1186/scrt5
dc.identifier.issn1574-888X
dc.identifier.issn1574-888X
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]
dc.identifier.urihttp://hdl.handle.net/2440/63822
dc.language.isoen
dc.publisherBentham Science Publishers Ltd.
dc.rightsCopyright ©2010 Yamaza et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1186/scrt5
dc.subjectBone Marrow Cells
dc.subjectMesenchymal Stem Cells
dc.subjectTooth, Deciduous
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectMice, Inbred MRL lpr
dc.subjectHumans
dc.subjectMice
dc.subjectMice, Nude
dc.subjectLupus Erythematosus, Systemic
dc.subjectTelomerase
dc.subjectLymphocyte Count
dc.subjectMesenchymal Stem Cell Transplantation
dc.subjectRegeneration
dc.subjectSignal Transduction
dc.subjectCell Proliferation
dc.subjectOsteogenesis
dc.subjectOdontogenesis
dc.subjectFemale
dc.subjectAdipogenesis
dc.subjectT-Lymphocytes, Regulatory
dc.subjectNeurogenesis
dc.subjectTh17 Cells
dc.subjectHeterografts
dc.titleImmunomodulatory properties of stem cells from human exfoliated deciduous teeth
dc.typeJournal article
pubs.publication-statusPublished

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