Characterization of a GSK-3 inhibitor in culture of human cord blood primitive hematopoietic cells
Date
2010
Authors
Jiang, J.
Zhao, M.
Zhang, A.
Yu, M.
Lin, X.
Wu, M.
Wang, X.
Lu, H.
Zhu, S.
Yu, Y.
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Journal article
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Biomedicine & Pharmacotherapy, 2010; 64(7):482-486
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Abstract
Wnt signaling pathway plays important roles in the biology of stem cells in maintaining their selfrenewal property. Glycogen synthase kinase-3 (GSK-3) inhibitors, the Wnt signaling agonists, maintain the pluripotency of embryonic stem cells. We report here that a synthetic GSK-3 inhibitor, 6- bromoindirubin-30-oxime (BIO), showed opposite effects on the expansion of human primitive hematopoietic cells isolated from umbilical cord blood (UCB). In combination with human c-kit ligand (KL), BIO at low concentration (0.2 mM) enhanced the expansion of UCB CD34+ cells, which was BIO structure and exposure time dependent; however, at high concentration (2 mM) it inhibited the expansion of the cells. Furthermore, hematopoietic stem cells (HSCs) were exhausted when the UCB CD34+ cells were exposed to 0.2 mM BIO and KL longer than 2 days. In conclusion, the use of BIO in expansion of UCB HSCs remains a significant challenge.
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Copyright 2010 Elsevier Masson SAS