Regulation of respiration in rotenone-treated tobacco cell suspension cultures
Date
2001
Authors
Zhang, Q.
Soole, K.
Wiskich, J.
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Advisors
Journal Title
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Journal article
Citation
Planta: an international journal of plant biology, 2001; 212(05-Jun):765-773
Statement of Responsibility
Qisen Zhang, Kathleen L. Soole and Joseph T. Wiskich
Conference Name
Abstract
Cells of Nicotiana tabacum L. suspension cultures were treated with the respiratory inhibitor rotenone, which specifically inhibits complex I activity of mitochondria. Rotenone retarded cell growth, as shown by decreases in fresh weight, dry weight and cell numbers on a suspension-volume basis. However, rates of the coupled respiration were higher in rotenone-treated compared to control cells when expressed on a fresh-weight basis. Rates of the rotenone-insensitive respiration increased substantially on both a fresh-weight and extractable-cellular-protein basis 24 h after rotenone treatment. ATP/ADP ratios were not significantly different between control and rotenone-treated cells. Our results indicated that cells of tobacco suspension cultures were able to maintain a slow rate of growth and adequate ATP/ADP ratios without the operation of complex I.
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The original publication can be found at www.springerlink.com