A short insert in the leader sequence of RNA 3L, a long variant of Alfalfa mosaic virus RNA3, introduces two unidentified reading frames

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dc.contributor.authorJayasena, K.
dc.contributor.authorRandles, J.
dc.date.issued2004
dc.descriptionThe original publication can be found at www.springerlink.com
dc.description.abstractN20-RNA 3L, a large form of RNA 3 associated with Alfalfa mosaic virus (AMV) strain N20 comprises 2281 nt and has approximately 97% overall sequence similarity to the longest previously described RNA 3 of AMV strain YSMV (YSMV-RNA 3; 2188 nt). Compared with YSMV-RNA 3, N20- RNA 3L contains an additional 97 nt in the 5’ leader upstream of the open reading frames for movement protein (MP) and coat protein (CP). Two overlapping unidentified reading frames (URF1 and URF2) result from this modification, each of which code for putative translation products of 21 amino acids. The URF1 putative peptide has a hydrophilic N-terminus and a hydrophobic C-terminus, indicating a possible association with both host cell membrane and cytosol whereas the putative URF2 product is predominantly hydrophobic. A further structural modification found in N20-RNA 3L is a new tandem repeat of 243 nts which overlaps with the MP open reading frame.
dc.description.statementofresponsibilityKithsiri W. Jayasena and John W. Randles
dc.identifier.citationVirus Genes, 2004; 29(3):311-316
dc.identifier.doi10.1007/s11262-004-7433-0
dc.identifier.issn0920-8569
dc.identifier.issn1572-994X
dc.identifier.orcidRandles, J. [0000-0003-2231-816X]
dc.identifier.urihttp://hdl.handle.net/2440/282
dc.language.isoen
dc.publisherKluwer Academic Publ
dc.source.urihttps://doi.org/10.1007/s11262-004-7433-0
dc.subjectAlfalfa mosaic virus-N20
dc.subjectRNA 3L
dc.subject5’ leader
dc.subjectURF1
dc.subjectURF2
dc.subject21aa peptides
dc.titleA short insert in the leader sequence of RNA 3L, a long variant of Alfalfa mosaic virus RNA3, introduces two unidentified reading frames
dc.typeJournal article
pubs.publication-statusPublished

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