Current studentsExisting staffContact us
 

Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo

Simple item page
dc.contributor.authorThorling, C.A.
dc.contributor.authorDancik, Y.
dc.contributor.authorHupple, C.W.
dc.contributor.authorMedley, G.
dc.contributor.authorLiu, X.
dc.contributor.authorZvyagin, A.V.
dc.contributor.authorRobertson, T.A.
dc.contributor.authorBurczynski, F.J.
dc.contributor.authorRoberts, M.S.
dc.date.issued2011
dc.description.abstractMultiphoton microscopy has been shown to be a useful tool in studying drug distribution in biological tissues. In addition, fluorescence lifetime imaging provides information about the structure and dynamics of fluorophores based on their fluorescence lifetimes. Fluorescein, a commonly used fluorescent probe, is metabolized within liver cells to fluorescein mono-glucuronide, which is also fluorescent. Fluorescein and its glucuronide have similar excitation and emission spectra, but different fluorescence lifetimes. In this study, we employed multiphoton fluorescence lifetime imaging to study the distribution and metabolism of fluorescein and its metabolite in vivo in rat liver. Fluorescence lifetime values in vitro were used to interpret in vivo data. Our results show that the mean fluorescence lifetimes of fluorescein and its metabolite decrease over time after injection of fluorescein in three different regions of the liver. In conclusion, we have demonstrated a novel method to study a fluorescent compound and metabolite in vivo using multiphoton fluorescence lifetime imaging.
dc.identifier.citationJournal of Biomedical Optics, 2011; 16(8):086013.1-086013.7
dc.identifier.doi10.1117/1.3614473
dc.identifier.issn1083-3668
dc.identifier.issn1560-2281
dc.identifier.urihttps://hdl.handle.net/1959.8/123883
dc.language.isoen
dc.publisherSPIE - International Society for Optical Engineering
dc.relation.fundingNHMRC
dc.rightsCopyright 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)
dc.source.urihttps://doi.org/10.1117/1.3614473
dc.subjectfluorescein
dc.subjectfluorescein mono-glucuronide
dc.subjectfluorescence lifetime imaging
dc.subjectliver
dc.subjectmetabolism
dc.subjectmultiphoton microscopy
dc.titleMultiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915909382901831

Files

Collections