Current studentsExisting staffContact us
 

Development of certified matrix-based reference material as a calibrator for genetically modified rice G6H1 analysis

Simple item page
dc.contributor.authorYang, Y.
dc.contributor.authorLi, L.
dc.contributor.authorYang, H.
dc.contributor.authorLi, X.
dc.contributor.authorZhang, X.
dc.contributor.authorXu, J.
dc.contributor.authorZhang, D.
dc.contributor.authorJin, W.
dc.contributor.authorYang, L.
dc.date.issued2018
dc.description.abstractThe accurate monitoring and quantification of genetically modified organisms (GMOs) are key points for the implementation of labeling regulations, and a certified reference material (CRM) acts as the scaleplate for quantifying the GM contents of foods/feeds and evaluating a GMO analytical method or equipment. Herein we developed a series of CRMs for transgenic rice event G6H1, which possesses insect-resistant and herbicide-tolerant traits. Three G6H1 CRMs were produced by mixing seed powders obtained from homozygous G6H1 and its recipient cultivar Xiushui 110 at mass ratios of 49.825%, 9.967%, and 4.986%. The between-bottle homogeneity and within-bottle homogeneity were thoroughly evaluated with consistent results. The potential DNA degradation in transportation and shelf life were evaluated with an expiration period of at least 12 months. The property values of three CRMs (G6H1a, G6H1b, G6H1c) were given as (49.825 ± 0.448) g/kg, (9.967 ± 1.757) g/kg, and (4.986 ± 1.274 g/kg based on mass fraction ratio, respectively. Furthermore, the three CRMs were characterized with values of (5.01 ± 0.08)%, (1.06 ± 0.22)%, and (0.53 ± 0.11)% based on the copy number ratio using the droplet digital PCR method. All results confirmed that the produced G6H1 matrix-based CRMs are of high quality with precise characterization values and can be used as calibrators in GM rice G6H1 inspection and monitoring and in evaluating new analytical methods or devices targeting the G6H1 event.
dc.description.statementofresponsibilityYu Yang, Liang Li, Hui Yang, Xiaying Li, Xiujie Zhang, Junfeng Xu, Dabing Zhang, Wujun Jin and Litao Yang
dc.identifier.citationJournal of Agricultural and Food Chemistry, 2018; 66(14):3708-3715
dc.identifier.doi10.1021/acs.jafc.8b00468
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.orcidZhang, D. [0000-0003-3181-9812]
dc.identifier.urihttp://hdl.handle.net/2440/116537
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.rights© 2018 American Chemical Society
dc.source.urihttps://doi.org/10.1021/acs.jafc.8b00468
dc.subjectGenetically modified organism; certified reference materials; G6H1 rice; characterization value
dc.titleDevelopment of certified matrix-based reference material as a calibrator for genetically modified rice G6H1 analysis
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Agriculture, Food and Wine publications