Antibody modified porous silicon microparticles for the selective capture of cells
Date
2014
Authors
Guan, B.
Magenau, A.
Ciampi, S.
Gaus, K.
Reece, P.J.
Gooding, J.J.
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Journal article
Citation
Bioconjugate Chemistry, 2014; 25(7):1282-1289
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Abstract
Herein, the ability of porous silicon (PSi) particles for selectively binding to specific cells is investigated. PSi microparticles with a high reflectance band in the reflectivity profile are fabricated, and subsequently passivated and modified with antibodies via the Cu(I)-catalyzed alkyne-azide cycloaddition reaction and succimidyl activation. To demonstrate the ability of the antibody-modified PSi particles to selectively bind to one cell type over others, HeLa cells were transfected with surface epitopes fused to fluorescent proteins. The antibody-functionalized PSi particles showed good selectivity for the corresponding surface protein on HeLa cells, with no significant cross-reactivity. The results are important for the application of PSi particles in cell sensing and drug delivery.
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Dissertation Note
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Data source: Supporting information, http://pubs.acs.org/doi/suppl/10.1021/bc500144u
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Copyright 2014 American Chemical Society