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Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae

Date

2020

Authors

Neville, S.L.
Eijkelkamp, B.A.
Lothian, A.
Paton, J.C.
Roberts, B.R.
Rosch, J.W.
McDevitt, C.A.

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Advisors

Journal Title

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Type:

Journal article

Citation

Communications Biology, 2020; 3(1):1-15

Statement of Responsibility

Stephanie L. Neville, Bart A. Eijkelkamp, Amber Lothian, James C. Paton, Blaine R. Roberts, Jason W. Rosch and Christopher A. McDevitt

Conference Name

DOI

10.1038/s42003-020-01417-y

Abstract

Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulation of metal ion homeostasis remain to be elucidated. Here, we used cadmium, a non-physiological metal ion, to investigate how the bacterial pathogen, Streptococcus pneumoniae, resists metal ion stress and dyshomeostasis. By combining transcriptomics, metabolomics and metalloproteomics, we reveal that cadmium stress dysregulates numerous essential cellular pathways including central carbon metabolism, lipid membrane biogenesis and homeostasis, and capsule production at the transcriptional and/or functional level. Despite the breadth of cellular pathways susceptible to metal intoxication, we show that S. pneumoniae is able to maintain viability by utilizing cellular pathways that are predominately metal-independent, such as the pentose phosphate pathway to maintain energy production. Collectively, this work provides insight into the cellular processes impacted by cadmium and how resistance to metal ion toxicity is achieved in S. pneumoniae.

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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Grant ID

http://purl.org/au-research/grants/nhmrc/1122582
 http://purl.org/au-research/grants/nhmrc/1080784
 http://purl.org/au-research/grants/nhmrc/1071659
 http://purl.org/au-research/grants/nhmrc/1142695
 http://purl.org/au-research/grants/arc/170102102
 http://purl.org/au-research/grants/nhmrc/1138673
 http://purl.org/au-research/grants/nhmrc/1061550
 http://purl.org/au-research/grants/arc/FT170100006

Published Version

https://doi.org/10.1038/s42003-020-01417-y

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Persistent link to this record

http://hdl.handle.net/2440/129930