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In vivo dual RNA-seq reveals that neutrophil recruitment underlies differential tissue tropism of Streptococcus pneumoniae

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dc.contributor.authorMinhas, V.
dc.contributor.authorAprianto, R.
dc.contributor.authorMcAllister, L.J.
dc.contributor.authorWang, H.
dc.contributor.authorDavid, S.C.
dc.contributor.authorMcLean, K.T.
dc.contributor.authorComerford, I.
dc.contributor.authorMcColl, S.R.
dc.contributor.authorPaton, J.C.
dc.contributor.authorVeening, J.-W.
dc.contributor.authorTrappetti, C.
dc.date.issued2020
dc.description.abstractStreptococcus pneumoniae is a genetically diverse human-adapted pathogen commonly carried asymptomatically in the nasopharynx. We have recently shown that a single nucleotide polymorphism (SNP) in the raffinose pathway regulatory gene rafR accounts for a difference in the capacity of clonally-related strains to cause localised versus systemic infection. Using dual RNA-seq, we show that this SNP affects expression of bacterial genes encoding multiple sugar transporters, and fine-tunes carbohydrate metabolism, along with extensive rewiring of host transcriptional responses to infection, particularly expression of genes encoding cytokine and chemokine ligands and receptors. The data predict a crucial role for differential neutrophil recruitment (confirmed by in vivo neutrophil depletion and IL-17 neutralization) indicating that early detection of bacteria by the host in the lung environment is crucial for effective clearance. Thus, dual RNA-seq provides a powerful tool for understanding complex host-pathogen interactions and reveals how a single bacterial SNP can drive differential disease outcomes.
dc.description.statementofresponsibilityVikrant Minhas, Rieza Aprianto, Lauren J. McAllister, Hui Wang, Shannon C. David, Kimberley T. McLean ... et al.
dc.identifier.citationCommunications Biology, 2020; 3(1):293-293
dc.identifier.doi10.1038/s42003-020-1018-x
dc.identifier.issn2399-3642
dc.identifier.issn2399-3642
dc.identifier.orcidMinhas, V. [0000-0002-2750-4677]
dc.identifier.orcidDavid, S.C. [0000-0003-3345-9443]
dc.identifier.orcidMcLean, K.T. [0000-0001-6743-2527]
dc.identifier.orcidMcColl, S.R. [0000-0003-0949-4660]
dc.identifier.orcidPaton, J.C. [0000-0001-9807-5278]
dc.identifier.orcidTrappetti, C. [0000-0001-8272-0068]
dc.identifier.urihttp://hdl.handle.net/2440/126244
dc.language.isoen
dc.publisherNature
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1071659
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1174876
dc.rightsOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttp://www.nature.com/
dc.subjectInfection; pathogens
dc.titleIn vivo dual RNA-seq reveals that neutrophil recruitment underlies differential tissue tropism of Streptococcus pneumoniae
dc.typeJournal article
pubs.publication-statusPublished

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