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Electrokinetic properties of lubricin antiadhesive coatings in microfluidic systems

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dc.contributor.authorGreene, G.W.
dc.contributor.authorDuffy, E.
dc.contributor.authorShallan, A.
dc.contributor.authorWuethrich, A.
dc.contributor.authorPaull, B.
dc.date.issued2016
dc.description.abstractLubricin is a glycoprotein found in articular joints which has long been recognized as being an important biological boundary lubricant molecule and, more recently, an impressive antiadhesive that readily self-assembles into a well ordered, polymer brush layer on virtually any substrate. The lubricin molecule possesses an overabundance of anionic charge, a property that is atypical among antiadhesive molecules, that enables its use as a coating for applications involving electrokinetic processes such as electrophoresis and electroosmosis. Coating the surfaces of silica and polymeric microfluidic devices with self-assembled lubricin coatings affords a unique combination of excellent fouling resistance and high charge density that enables notoriously "sticky" biomolecules such as proteins to be used and controlled electrokinetically in the device without complications arising from nonspecific adsorption. Using capillary electrophoresis, we characterized the stability, uniformity, and electrokinetic properties of lubricin coatings applied to silica and PTFE capillaries over a range of run buffer pHs and when exposed to concentrated solutions of protein. In addition, we demonstrate the effectiveness of lubricin as a coating to minimize nonspecific protein adsorption in an electrokinetically controlled polydimethylsiloxane/silica microfluidic device.
dc.identifier.citationLangmuir, 2016; 32(7):1899-1908
dc.identifier.doi10.1021/acs.langmuir.5b03535
dc.identifier.issn0743-7463
dc.identifier.issn1520-5827
dc.identifier.urihttps://hdl.handle.net/11541.2/132415
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.fundingARC DE130101458
dc.rightsCopyright 2016 American Chemical Society
dc.source.urihttp://doi.org/10.1021/acs.langmuir.5b03535
dc.subjectmicrofluidic systems
dc.subjectlubricin
dc.titleElectrokinetic properties of lubricin antiadhesive coatings in microfluidic systems
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916211004801831

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