Peptide-based star polymers as potential siRNA carriers
Date
2014
Authors
Lam, S.J.
Sulistio, A.
Ladewig, K.
Wong, E.H.H.
Blencowe, A.
Qiao, G.G.
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Journal article
Citation
Australian Journal of Chemistry, 2014; 67(4):592-597
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Abstract
<jats:p>16- and 32-arm star polymers were synthesised using poly(amido amine) (PAMAM) dendrimers as multifunctional initiators for the ring-opening polymerisation (ROP) of ϵ-Z-l-lysine N-carboxyanhydride (Lys NCA) via the core-first approach. The resulting star polymers were subsequently post-functionalised with poly(ethylene glycol) (PEG) via carbodiimide coupling, potentially improving the biodistribution of the stars in vivo. De-protection of the carboxybenzyl (Cbz)-protected star arms yielded water-soluble cationic poly(l-lysine) (PLL) star polymers with hydrodynamic radii ranging from 2.0 to 3.3 nm. Successful complexation of the PLL star polymers with double-stranded oligodeoxynucleotides (ODNs)—a mimic for small interfering RNA (siRNA)—was achieved at a nitrogen-to-phosphate (N/P) ratio of 5. Cell viability studies using HEK293T cells indicated the ‘safe’ concentration for these polymers is within a suitable window for the delivery of siRNA therapeutics.</jats:p>
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Link to a related website: http://pdfs.semanticscholar.org/ddc7/dd961946ee881c9f4df2cea6e3ecc69f427b.pdf, Open Access via Unpaywall
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Copyright 2014 CSIRO