The solution structure of frenatin 3, a neuronal nitric oxide synthase inhibitor from the giant tree frog, Litoria infrafrenata
| dc.contributor.author | Brinkworth, C. | |
| dc.contributor.author | Carver, J. | |
| dc.contributor.author | Wegener, K. | |
| dc.contributor.author | Doyle, J. | |
| dc.contributor.author | Llewellyn, L. | |
| dc.contributor.author | Bowie, J. | |
| dc.date.issued | 2003 | |
| dc.description | The definitive version may be found at www.wiley.com | |
| dc.description.abstract | The peptide frenatin 3 is a major component of the skin secretion of the Australian giant tree frog, Litoria infrafrenata. Frenatin 3 is 22 amino acids in length, and shows neither antimicrobial nor anticancer activity. It inhibits the production of nitric oxide by the enzyme neuronal nitric oxide synthase at a micromolar concentration by binding to its regulatory protein, Ca2+ calmodulin, a protein known to recognize and bind amphipathic alpha-helices. The solution structure of frenatin 3 has been investigated using NMR spectroscopy and restrained molecular dynamics calculations. In trifluoroethanol/water mixtures, the peptide forms an amphipathic alpha-helix over residues 1-14 while the C-terminal eight residues are more flexible and less structured. The flexible region may be responsible for the lack of antimicrobial activity. In water, frenatin 3 exhibits some alpha-helical character in its N-terminal region. | |
| dc.description.statementofresponsibility | Craig S. Brinkworth, John A. Carver, Kate L. Wegener, Jason Doyle, Lyndon E. Llewellyn and John H. Bowie | |
| dc.identifier.citation | Biopolymers, 2003; 70(3):424-434 | |
| dc.identifier.doi | 10.1002/bip.10524 | |
| dc.identifier.issn | 0006-3525 | |
| dc.identifier.issn | 1097-0282 | |
| dc.identifier.orcid | Wegener, K. [0000-0002-1562-6060] | |
| dc.identifier.uri | http://hdl.handle.net/2440/39363 | |
| dc.language.iso | en | |
| dc.provenance | Published Online: 2 Oct 2003 | |
| dc.publisher | John Wiley & Sons Inc | |
| dc.source.uri | http://www3.interscience.wiley.com/journal/105561429/abstract | |
| dc.subject | amphibian peptides | |
| dc.subject | neuronal nitric oxide synthase inhibition | |
| dc.subject | NMR spectroscopy | |
| dc.subject | solution structure | |
| dc.title | The solution structure of frenatin 3, a neuronal nitric oxide synthase inhibitor from the giant tree frog, Litoria infrafrenata | |
| dc.type | Journal article | |
| pubs.publication-status | Published |