Analysis of infection time courses shows CII levels determine the frequency of lysogeny in phage 186

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dc.contributor.authorHao, N.
dc.contributor.authorAgnew, D.
dc.contributor.authorKrishna, S.
dc.contributor.authorDodd, I.B.
dc.contributor.authorShearwin, K.E.
dc.date.issued2021
dc.descriptionPublished: 29 September 2021
dc.description.abstractEngineered phage with properties optimised for the treatment of bacterial infections hold great promise, but require careful characterisation by a number of approaches. Phage–bacteria infection time courses, where populations of bacteriophage and bacteria are mixed and followed over many infection cycles, can be used to deduce properties of phage infection at the individual cell level. Here, we apply this approach to analysis of infection of Escherichia coli by the temperate bacteriophage 186 and explore which properties of the infection process can be reliably inferred. By applying established modelling methods to such data, we extract the frequency at which phage 186 chooses the lysogenic pathway after infection, and show that lysogenisation increases in a graded manner with increased expression of the lysogenic establishment factor CII. The data also suggest that, like phage λ, the rate of lysogeny of phage 186 increases with multiple infections.
dc.description.statementofresponsibilityNan Hao, Dylan Agnew, Sandeep Krishna, Ian B. Dodd and Keith E. Shearwin
dc.identifier.citationPharmaceuticals, 2021; 14(10):998-1-998-14
dc.identifier.doi10.3390/ph14100998
dc.identifier.issn1424-8247
dc.identifier.issn1424-8247
dc.identifier.orcidHao, N. [0000-0001-5836-3507]
dc.identifier.orcidDodd, I.B. [0000-0003-2969-6841]
dc.identifier.orcidShearwin, K.E. [0000-0002-7736-2742]
dc.identifier.urihttps://hdl.handle.net/2440/133008
dc.language.isoen
dc.publisherMDPI AG
dc.relation.granthttp://purl.org/au-research/grants/arc/DE150100091
dc.relation.granthttp://purl.org/au-research/grants/arc/DP150103009
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
dc.source.urihttps://doi.org/10.3390/ph14100998
dc.subjectlysogeny; phage infection; multiplicity of infection; bacteriophage; temperate phage; phage therapy; CII protein; synthetic biology
dc.titleAnalysis of infection time courses shows CII levels determine the frequency of lysogeny in phage 186
dc.typeJournal article
pubs.publication-statusPublished

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