Cloning and analysis of the human complement factor H gene promoter

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dc.contributor.authorWard, H.
dc.contributor.authorHiggs, N.
dc.contributor.authorBlackmore, T.
dc.contributor.authorSadlon, T.
dc.date.issued1997
dc.description.abstractThe 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements, cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.
dc.description.statementofresponsibilityHelena M Ward, Naomi H Higgs, Timothy K Blackmore, Tania A Sadlon and David L Gordon
dc.identifier.citationImmunology and Cell Biology, 1997; 75(5):508-510
dc.identifier.doi10.1038/icb.1997.79
dc.identifier.issn0818-9641
dc.identifier.issn1440-1711
dc.identifier.orcidWard, H. [0000-0002-3831-1205]
dc.identifier.urihttp://hdl.handle.net/2440/81789
dc.language.isoen
dc.publisherNature Publishing Group
dc.rightsCopyright status unknown
dc.source.urihttps://doi.org/10.1038/icb.1997.79
dc.subjectCloning
dc.subjectComplement
dc.subjectGene promoter
dc.titleCloning and analysis of the human complement factor H gene promoter
dc.typeJournal article
pubs.publication-statusPublished

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