Cell-free synthesis of a functional membrane transporter into a tethered bilayer lipid membrane
Date
2016
Authors
Zieleniecki, J.
Nagarajan, Y.
Waters, S.
Rongala, J.
Thompson, V.
Hrmova, M.
Köper, I.
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Journal article
Citation
Langmuir, 2016; 32(10):2445-2449
Statement of Responsibility
Julius L. Zieleniecki, Yagnesh Nagarajan, Shane Waters, Jay Rongala, Vanessa Thompson, Maria Hrmova, and Ingo Köper
Conference Name
Abstract
Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.
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© 2016 American Chemical Society