Tissue-specific regulation of cyclin E transcription during Drosophila melanogaster embryogenesis
| dc.contributor.author | Jones, L. | |
| dc.contributor.author | Richardson, H. | |
| dc.contributor.author | Saint, R. | |
| dc.contributor.organisation | Centre for the Molecular Genetics of Development | |
| dc.date.issued | 2000 | |
| dc.description.abstract | Cyclin E is an essential regulator of S phase entry. We have previously shown that transcriptional regulation of the gene that encodes Drosophila cyclin E, DmcycE, plays an important role in the control of the G(1) to S phase transition during development. We report here the first comprehensive analysis of the transcriptional regulation of a G(1 )phase cell cycle regulatory gene during embryogenesis. Analysis of deficiencies, a genomic transformant and reporter gene constructs revealed that DmcycE transcription is controlled by a large and complex cis-regulatory region containing tissue- and stage-specific components. Separate regulatory elements for transcription in epidermal cells during cell cycles 14-16, central nervous system cells and peripheral nervous system cells were found. An additional cis-regulatory element drives transcription in thoracic epidermal cells that undergo a 17th cell cycle when other epidermal cells have arrested in G(1 )phase prior to terminal differentiation. The complexity of DmcycE transcriptional regulation argues against a model in which DmcycE transcription is regulated simply and solely by G(1) to S phase transcription regulators such as RB, E2F and DP. Rather, our study demonstrates that tissue-specific transcriptional regulatory mechanisms are important components of the control of cyclin E transcription and thus of cell proliferation in metazoans. | |
| dc.description.statementofresponsibility | Lynn Jones, Helena Richardson and Robert Saint | |
| dc.identifier.citation | Development, 2000; 127(21):4619-4630 | |
| dc.identifier.doi | 10.1242/dev.127.21.4619 | |
| dc.identifier.issn | 0950-1991 | |
| dc.identifier.issn | 1477-9129 | |
| dc.identifier.uri | http://hdl.handle.net/2440/28002 | |
| dc.language.iso | en | |
| dc.publisher | Company of Biologists Ltd | |
| dc.rights | © The Company of Biologists Limited 2000 | |
| dc.source.uri | http://dev.biologists.org/cgi/content/abstract/127/21/4619 | |
| dc.subject | Drosophila | |
| dc.title | Tissue-specific regulation of cyclin E transcription during Drosophila melanogaster embryogenesis | |
| dc.type | Journal article | |
| pubs.publication-status | Published |
Files
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- hdl_28002.pdf
- Size:
- 518.67 KB
- Format:
- Adobe Portable Document Format