Effects of Ca2+ concentration on GABAB receptor function in rat neocortical slices

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dc.contributor.authorOng, J.
dc.contributor.authorKerr, D.
dc.date.issued2000
dc.description.abstractIn rat neocortical slices maintained in Mg2+-free Kreb's medium, the effects of Ca2+ concentration on repetitive spontaneous discharges and on GABA(B) receptor-mediated responses were investigated. Over a concentration range of 0.3-2.4 mM Ca2+, there was a reduction of discharge amplitude, with a 50 +/- 6.5% reduction in 0.3 mM Ca2+, whilst the burst frequency remained unaffected. Baclofen, the GABA(B) receptor agonist, produced a concentration-dependent depression of discharge frequency, reversibly antagonised by the antagonist (+)-(S)-5,5-dimethylmorpholinyl-2-acetic acid (Sch 50911). The EC50 value for baclofen in 2.4 mM Ca2+ was 11 microM, while the EC50 values in 0.3, 0.6, 1.2, and 1.8 mM Ca2+ were 1.3, 2.5, 3.6, and 10 microM, respectively, resulting in 8.5, 4.4, 3.1, and 1.1-fold leftward shifts. This enhanced action of baclofen in low extracellular Ca2+ concentrations in the neocortex may be the result of a lower concentration gradient which reinforces the action of baclofen.
dc.identifier.citationCanadian Journal of Physiology and Pharmacology, 2000; 78(6):507-511
dc.identifier.doi10.1139/y00-017
dc.identifier.issn0008-4212
dc.identifier.issn1205-7541
dc.identifier.orcidOng, J. [0000-0002-0958-460X]
dc.identifier.urihttp://hdl.handle.net/2440/14740
dc.language.isoen
dc.publisherNatl Research Council Canada
dc.subjectNeocortex
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectCalcium Chloride
dc.subjectBaclofen
dc.subjectReceptors, GABA-B
dc.subjectGABA Agonists
dc.subjectSynaptic Transmission
dc.subjectMale
dc.titleEffects of Ca2+ concentration on GABAB receptor function in rat neocortical slices
dc.typeJournal article
pubs.publication-statusPublished

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