Validation of molecular markers for wheat breeding

Date

2001

Authors

Sharp, P.
Johnston, S.
Brown, G.
McIntosh, R.
Pallotta, M.
Carter, M.
Bariana, H.
Khatkar, S.
Lagudah, E.
Singh, R.

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Crop and Pasture Science, 2001; 52(11-Dec):1357-1366

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P. J. Sharp, S. Johnston, G. Brown, R. A. McIntosh, M. Pallotta, M. Carter, H. S. Bariana, S. Khatkar, E. S. Lagudah, R. P. Singh, M. Khairallah, R. Potter and M. G. K. Jones

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Abstract

<jats:p>Five sets of markers were assessed for their usefulness in breeding, two linked to wheat stem rust gene Sr2 , several markers linked to a chromosome segment conferring Yr17/Lr37/Sr38 resistance, two reported markers for the linked genes Lr35 and Sr39 , one for Lr28 , and one linked to flour colour. The gene for Sr2 confers adult plant resistance to stem rust ( Puccinia graminis f.sp. tritici ) and was originally transferred to bread wheat from the tetraploid emmer (‘Yaroslav’) to the cultivars Hope and H-44. The gene is located on the short arm of chromosome 3B and confers a durable adult plant resistance to stem rust usually expressed only in the field. The chromosome segment carrying the Lr37 , Sr38 , Yr17 resistance genes is located on 2AS and was originally introduced into wheat through an Aegilops ventricosa Triticum persicum cross, followed by a cross to the cultivar Marne (VPM1). The flour colour quantitative trait locus was originally described in a Yarralinka Schomburg cross and is located on chromosome 7A. The primers as originally developed required optimisation for more routine use in a breeding program.</jats:p>

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