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Host defence peptides from the skin glands of the Australian Blue Mountains tree-frog Litoria citropa

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dc.contributor.authorWegener, K.
dc.contributor.authorWabnitz, P.
dc.contributor.authorCarver, J.
dc.contributor.authorBowie, J.
dc.contributor.authorChia, B.
dc.contributor.authorWallace, J.
dc.contributor.authorTyler, M.
dc.date.issued1999
dc.description.abstractNineteen citropin peptides are present in the secretion from the granular dorsal glands of the Blue Mountains tree-frog Litoria citropa; 15 of these peptides are also present in the secretion from the submental gland. Two major peptides, citropin 1.1 (GLFDVIKKVASVIGGL-NH2), citropin 1.2 (GLFDIIKKVASVVGGL-NH2) and a minor peptide, citropin 1.3 (GLFDIIKKVASVIGGL-NH2) are wide-spectrum antibacterial peptides. The amphibian has an endoprotease which deactivates these membrane-active peptides by removing residues from the N-terminal end: loss of three residues gives the most abundant degradation products. The solution structure of the basic peptide citropin 1.1 has been determined by NMR spectroscopy [in a solvent mixture of trifluoroethanol/water (1 : 1)] to be an amphipathic alpha-helix with well-defined hydrophobic and hydrophilic regions. The additional four peptides produced by the dorsal glands are structurally related to the antibacterial citropin 1 peptides but contain three more residues at their C-terminus [e.g. citropin 1.1.3 (GLFDVIKKVASVIGLASP-OH)]. These peptides show minimal antibacterial activity; their role in the amphibian skin is not known.
dc.identifier.citationThe Federation of European Biochemical Societies (FEBS) Journal, 1999; 265(2):627-637
dc.identifier.doi10.1046/j.1432-1327.1999.00750.x
dc.identifier.issn0014-2956
dc.identifier.issn1432-1033
dc.identifier.orcidWegener, K. [0000-0002-1562-6060]
dc.identifier.urihttp://hdl.handle.net/2440/12153
dc.language.isoen
dc.publisherWILEY-BLACKWELL
dc.source.urihttps://doi.org/10.1046/j.1432-1327.1999.00750.x
dc.subjectSkin
dc.subjectAnimals
dc.subjectAnura
dc.subjectPeptides
dc.subjectAntimicrobial Cationic Peptides
dc.subjectProteins
dc.subjectAmphibian Proteins
dc.subjectAnti-Infective Agents
dc.subjectCircular Dichroism
dc.subjectMagnetic Resonance Spectroscopy
dc.subjectSequence Analysis
dc.subjectAmino Acid Sequence
dc.subjectModels, Molecular
dc.subjectMolecular Sequence Data
dc.subjectMass Spectrometry
dc.titleHost defence peptides from the skin glands of the Australian Blue Mountains tree-frog Litoria citropa
dc.typeJournal article
pubs.publication-statusPublished

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