Multiphoton microscopy applications in biology

Date

2014

Authors

Yamada, M.
Lin, L.L.
Prow, T.W.

Editors

Cornea, A.
Conn, P.M.

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Book chapter

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Source details - Title: Fluorescence Microscopy: Super-Resolution and other Novel Techniques, 2014 / Cornea, A., Conn, P.M. (ed./s), Ch.13, pp.185-197

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Abstract

This chapter provides an overview of basic principles of multiphoton microscopy, including a brief summary of how multiphoton microscopy fits within the arena of fluorescent microscopy techniques. The description of how multiphoton microscopy works is geared toward both the novice and experienced user. There are several examples of how multiphoton microscopy can be applied to the life sciences. Each example has a detailed section on the configuration of the device in context with the study focus. These applications include tracking fluorescent protein-expressing immune cells, drug-containing nanoparticles, metal nanoparticles, multiphoton microscopy, microendoscopy, and clinical multiphoton microscopy. Finally, the limitations and future of multiphoton microscopy are explored.

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Copyright 2014 Elsevier

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