Long-term pulmonary effects of intrauterine exposure to endotoxin following preterm birth in sheep
Date
2012
Authors
Atik, A.
Sozo, F.
Orgeig, S.
Suri, L.N.M.
Hanita, T.
Harding, R.
De, M.R.
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Journal article
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Reproductive Sciences, 2012; 19(12):1352-1364
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Abstract
Our aim was to determine whether fetal exposure to intraamniotic lipopolysaccharide (LPS) persistently alters the lungs following moderate preterm birth. Fetal sheep were exposed to LPS (1 mg/d) or saline from 0.75 to preterm birth at 0.90 of gestation. Eleven weeks after preterm birth, lung structure was unaltered. Interleukin (IL)-1b messenger RNA (mRNA) levels were elevated in lungs of LPS-exposed lambs (P < .05) but IL-1b protein levels were unaltered. Lung mRNA levels of IL-6, IL-8 and tumor necrosis factor a, and percentage of inflammatory cells were not different between groups. Surfactant protein (SP)-A and SP-C mRNA levels and SP-B tissue protein expression were higher in LPS-exposed lambs than controls (all P < .05); however, expression of SP-A and SP-C proteins was reduced. Prenatal LPS exposure causes a persistent increase in gene expression of proinflammatory mediators and surfactant proteins and a decrease in lung tissue SP-A and -C protein expression after preterm birth, which may affect lung immunity.
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Copyright 2012 The Authors