Atomic force microscopy and spectroscopic studies of crosslinking induced changes in the extracellular matrix that influence nanomechanical properties /
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(Published version)
Date
2019
Authors
Le Cerf, Brock
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thesis
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Abstract
The tissue microenvironment has received significant attention in recent decades since the revelation that it contains biophysical and biochemical cues that influence critical cellular behaviour such as proliferation,differentiation, migrations and survival. In this thesis, atomic force microscopy is used on a simple type I collagen model. The data shows that current assumptions in the literature attributing the bimodal distribution of modulus values found in tissue to proteoglycan and collagen may not be valid. The data suggests that the bimodal values are both related to collagen. The effect of glutaraldehyde cross-linking on the nanomechanical profile was examined, revealing a significant distortion. Infrared spectroscopy was used to further investigate the changes of the chemical environment due to cross-linking.
School/Discipline
University of South Australia. Future Industries Institute.
Future Industries Institute.
Future Industries Institute.
Dissertation Note
Thesis (Masters by research(Biomaterials Engineering and Nanomedicine))--University of South Australia, 2019.
Provenance
Copyright 2019 Brock A. Le Cerf.
Description
1 ethesis (xiii, 138 pages) :
colour illustrations.
Includes bibliographical references (pages 119-122)
colour illustrations.
Includes bibliographical references (pages 119-122)
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506 0#$fstar $2Unrestricted online access