Expression profiling reveals functionally important genes and coordinately regulated signaling pathway genes during in vitro angiogenesis

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dc.contributor.authorHahn, C.
dc.contributor.authorSu, Z.
dc.contributor.authorDrogemuller, C.
dc.contributor.authorTsykin, A.
dc.contributor.authorWaterman, S.
dc.contributor.authorBrautigan, P.
dc.contributor.authorYu, S.
dc.contributor.authorKremmidiotis, G.
dc.contributor.authorGardner, A.
dc.contributor.authorSolomon, P.
dc.contributor.authorGoodall, G.
dc.contributor.authorVadas, M.
dc.contributor.authorGamble, J.
dc.date.issued2005
dc.description.abstractAngiogenesis is a complex multicellular process requiring the orchestration of many events including migration, alignment, proliferation, lumen formation, remodeling, and maturation. Such complexity indicates that not only individual genes but also entire signaling pathways will be crucial in angiogenesis. To define an angiogenic blueprint of regulated genes, we utilized our well-characterized three-dimensional collagen gel model of in vitro angiogenesis, in which the majority of cells synchronously progress through defined morphological stages culminating in the formation of capillary tubes. We developed a comprehensive three-tiered approach using microarray analysis, which allowed us to identify genes known to be involved in angiogenesis and genes hitherto unlinked to angiogenesis as well as novel genes and has proven especially useful for genes where the magnitude of change is small. Of interest is the ability to recognize complete signaling pathways that are regulated and genes clustering into ontological groups implicating the functional importance of particular processes. We have shown that consecutive members of the mitogen-activated protein kinase and leukemia inhibitory factor signaling pathways are altered at the mRNA level during in vitro angiogenesis. Thus, at least for the mitogen-activated protein kinase pathway, mRNA changes as well as the phosphorylation changes of these gene products may be important in the control of blood vessel morphogenesis. Furthermore, in this study, we demonstrated the power of virtual Northern blot analysis, as an alternative to quantitative RT-PCR, for measuring the magnitudes of differential gene expression.
dc.description.statementofresponsibilityC. N. Hahn, Z. J. Su, C. J. Drogemuller, A. Tsykin, S. R. Waterman, P. J. Brautigan, S. Yu, G. Kremmidiotis, A. Gardner, P. J. Solomon, G. J. Goodall, M. A. Vadas, and J. R. Gamble
dc.identifier.citationPhysiological Genomics, 2005; 22(1):57-69
dc.identifier.doi10.1152/physiolgenomics.00278.2004
dc.identifier.issn1094-8341
dc.identifier.issn1531-2267
dc.identifier.orcidHahn, C. [0000-0001-5105-2554]
dc.identifier.orcidDrogemuller, C. [0000-0001-9770-4845]
dc.identifier.orcidGardner, A. [0009-0009-7321-1697]
dc.identifier.orcidSolomon, P. [0000-0002-0667-6947]
dc.identifier.orcidGoodall, G. [0000-0003-1294-0692]
dc.identifier.urihttp://hdl.handle.net/2440/17826
dc.language.isoen
dc.publisherAmerican Physiological Society
dc.rights© 2005, The American Physiological Society
dc.source.urihttps://doi.org/10.1152/physiolgenomics.00278.2004
dc.subjectGene expression
dc.subjectmitogen-activated protein kinase pathway
dc.titleExpression profiling reveals functionally important genes and coordinately regulated signaling pathway genes during in vitro angiogenesis
dc.typeJournal article
pubs.publication-statusPublished

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