The systematics of the culex sitiens Wiedemann (diptera:culicidae) subgroup species complex /

Date

2005

Authors

Kent, Joanne Kristina.

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thesis

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Abstract

Culex annulirostris Skuse (Diptera: Culicidae), the common banded mosquito, is the most important mosquito vector of arboviruses in Australia (Chapman et al. 2000; Mottram et al. 1986; Kay et al. 1984; Liehne et al. 1981; Doherty et al. 1979; Doherty 1972; Standfast 1968). Culex annulirostris belongs to the Cx sitiens Wiedemann subgroup species complex.The main objectives were to first elucidate species boundaries of the Cx sitiens subgroup species complex via molecular data and to review morphological characters. This first aim constituted a two -tiered objective: 1) to examine whether a molecular diagnostic could be developed to separate species within the Cx sitiens subgroup species complex; and 2) to determine the presence or absence of cryptic species within the complex using allozymeelectrophoresis and the mitochondrial (mt) cytochrome oxidase subunit II (COB) gene. A review of morphological characteristics would show information regarding variations of characteristics that needed to be included in identification keys. The second main objective was to carry out a pilot population study of Cx annulirostris throughout Australia, the Torres Strait and Papua New Guinea using microsatellites. This involved the construction of amicro satellite library and development of microsatellite primers to examine genetic structureof Cx annulirostris with the intention of their being used in future population studies and possibly being used on other Culex species.

School/Discipline

University of South Australia. School of Pharmacy and Medical Sciences.
School of Pharmacy and Medical Sciences.

Dissertation Note

Thesis (PhD BiomedicalScience)--University of South Australia, 2005.

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Copyright 2005 Joanne Kent.

Description

1 ethesis (267 pages) :
illustrations (some colour), maps.
Includes bibliographical references: pages 129-158.

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506 0#$fstar $2Unrestricted online access

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