Transition from a maternal to external nitrogen source in maize seedlings

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2017

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Sabermanesh, K.
Holtham, L.
George, J.
Roessner, U.
Boughton, B.
Heuer, S.
Tester, M.
Plett, D.
Garnett, T.

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Journal of Integrative Plant Biology, 2017; 59(4):261-274

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Kasra Sabermanesh, Luke R. Holtham, Jessey George, Ute Roessner, Berin A. Boughton, Sigrid Heuer, Mark Tester, Darren C. Plett and Trevor P. Garnett

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Abstract

Maximizing NO₃¯ uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO₃¯ uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO₃¯ uptake and metabolism, to gain an understanding of how the NO₃¯ uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seed-derived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilizing eight days after imbibition (DAI). Similarly, shoot N% decreases, but does not stabilize until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO₃¯ uptake capacity increases until shoot N% stabilizes. The increase in root NO₃¯ uptake capacity corresponds with a rapid rise in transcript levels of putative NO₃¯ transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO₃¯ uptake capacity to meet N demand provide an insight into the processes controlling N uptake.

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© 2017 The Authors. Journal of Integrative Plant Biology Published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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