QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine

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dc.contributor.authorBartle, L.
dc.contributor.authorPeltier, E.
dc.contributor.authorSundstrom, J.F.
dc.contributor.authorSumby, K.
dc.contributor.authorMitchell, J.G.
dc.contributor.authorJiranek, V.
dc.contributor.authorMarullo, P.
dc.date.issued2021
dc.description.abstractThe two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions.
dc.description.statementofresponsibilityLouise Bartle, Emilien Peltier, Joanna F. Sundstrom, Krista Sumby, James G. Mitchell, Vladimir Jiranek, Philippe Marullo
dc.identifier.citationApplied Microbiology and Biotechnology, 2021; 105(12):5053-5066
dc.identifier.doi10.1007/s00253-021-11376-x
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.orcidBartle, L. [0000-0001-7112-1801]
dc.identifier.orcidSundstrom, J.F. [0000-0002-4898-3101]
dc.identifier.orcidSumby, K. [0000-0002-1833-2025]
dc.identifier.orcidJiranek, V. [0000-0002-9775-8963]
dc.identifier.urihttp://hdl.handle.net/2440/131090
dc.language.isoen
dc.publisherSpringer-Verlag
dc.relation.granthttp://purl.org/au-research/grants/arc/IC170100008
dc.rights© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021
dc.source.urihttps://doi.org/10.1007/s00253-021-11376-x
dc.subjectYeast-bacteria interactions; malolactic fermentation; QTL; wine; SSU1
dc.titleQTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine
dc.typeJournal article
pubs.publication-statusPublished

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