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Synthesis of peptide sequences derived from fibril-forming proteins

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dc.contributor.authorScanlon, D.
dc.contributor.authorKaras, J.
dc.contributor.editorHill, A.F.
dc.contributor.editorBarnham, K.J.
dc.contributor.editorBottomley, S.P.
dc.contributor.editorCappai, R.
dc.contributor.schoolSchool of Chemistry and Physics
dc.date.issued2011
dc.descriptionAlso published as a book chapter: Protein Folding, Misfolding, and Disease: Methods and Protocols, 2011 / A. F. Hill, K. J. Barnham, S. P. Bottomley & R. Cappai (eds.), Ch.3 pp.29-43
dc.description.abstractThe pathogenesis of a large number of diseases, including Alzheimer's Disease, Parkinson's Disease, and Creutzfeldt-Jakob Disease (CJD), is associated with protein aggregation and the formation of amyloid, fibrillar deposits. Peptide fragments of amyloid-forming proteins have been found to form fibrils in their own right and have become important tools for unlocking the mechanism of amyloid fibril formation and the pathogenesis of amyloid diseases. The synthesis and purification of peptide sequences derived from amyloid fibril-forming proteins can be extremely challenging. The synthesis may not proceed well, generating a very low quality crude product which can be difficult to purify. Even clean crude peptides can be difficult to purify, as they are often insoluble or form fibrils rapidly in solution. This chapter presents methods to recognise and to overcome the difficulties associated with the synthesis, and purification of fibril-forming peptides, illustrating the points with three synthetic examples.
dc.description.statementofresponsibilityDenis B. Scanlon and John A. Karas
dc.identifier.citationMethods in Molecular Biology, 2011; 752:29-43
dc.identifier.doi10.1007/978-1-60327-223-0_3
dc.identifier.isbn9781603272216
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttp://hdl.handle.net/2440/71365
dc.language.isoen
dc.publisherHumana Press, Inc.
dc.rights© Springer Science+Business Media, LLC 2011
dc.source.urihttps://doi.org/10.1007/978-1-60327-223-0_3
dc.subjectAmyloid
dc.subjectResins, Synthetic
dc.subjectPeptide Fragments
dc.subjectPrions
dc.subjectFreeze Drying
dc.subjectChromatography
dc.subjectMicrowaves
dc.subjectApolipoprotein C-II
dc.subjectAnalytic Sample Preparation Methods
dc.subjectAmyloid beta-Peptides
dc.titleSynthesis of peptide sequences derived from fibril-forming proteins
dc.typeJournal article
pubs.publication-statusPublished

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