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Isolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype

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dc.contributor.authorPerruchon, C.
dc.contributor.authorChatzinotas, A.
dc.contributor.authorOmirou, M.
dc.contributor.authorVasileiadis, S.
dc.contributor.authorMenkissoglou Spiroudi, U.
dc.contributor.authorKarpouzas, D.G.
dc.date.issued2017
dc.descriptionData source: Supplementary material, https://link-springer-com.access.library.unisa.edu.au/article/10.1007/s00253-017-8128-5#SupplementaryMaterial
dc.description.abstractThiabendazole (TBZ) is a fungicide used in fruit-packaging plants. Its application leads to the production of wastewaters requiring detoxification. In the absence of efficient treatment methods, biological depuration of these effluents could be a viable alternative. However, nothing is known regarding the microbial degradation of the recalcitrant and toxic to aquatics TBZ. We report the isolation, via enrichment cultures from a polluted soil, of the first bacterial consortium able to rapidly degrade TBZ and use it as a carbon source. Repeated efforts using various culture-dependent approaches failed to isolate TBZ-degrading bacteria in axenic cultures. Denaturating gradient gel electrophoresis (DGGE) and cloning showed that the consortium was composed of α-, β- and γ-Proteobacteria. Culture-independent methods including antibiotics-driven selection with DNA/RNA-DGGE, q-PCR and stable isotope probing (SIP)-DGGE identified a Sphingomonas phylotype (B13) as the key degrading member. Cross-feeding studies with structurally related chemicals showed that ring substituents of the benzimidazole moiety (thiazole or furan rings) favoured the cleavage of the imidazole moiety. LC-MS/MS analysis verified that TBZ degradation proceeds via cleavage of the imidazole moiety releasing thiazole-4-carboxamidine, which was not further transformed, and the benzoyl moiety, possibly as catechol, which was eventually consumed by the bacterial consortium as suggested by SIP-DGGE.
dc.identifier.citationApplied Microbiology and Biotechnology, 2017; 101(9):3881-3893
dc.identifier.doi10.1007/s00253-017-8128-5
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.urihttps://hdl.handle.net/11541.2/124788
dc.language.isoen
dc.publisherSpringer
dc.relation.fundingEuropean Social Fund and National Resources 4718
dc.relation.fundingState Scholarship Foundation of Greece (IKY)
dc.rightsCopyright 2017 Springer
dc.source.urihttps://doi.org/10.1007/s00253-017-8128-5
dc.subjectthiabendazole
dc.subjectfruit-packaging industry
dc.subjectwastewaters
dc.subjectSIP-DGGE
dc.subjectpesticide biodegradation
dc.subjectSphingomonas
dc.titleIsolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916113210701831

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