A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania

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dc.contributor.authorKelly, J.
dc.contributor.authorWard, H.
dc.contributor.authorMiles, M.
dc.contributor.authorKendall, G.
dc.date.issued1992
dc.description.abstractA Trypanosoma cruzi expression vector has been constructed using sequences derived from the flanking regions of the glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) genes. The neomycln phosphotransferase (neor) gene was incorporated as a selectable marker. Using electroporatlon we have introduced this vector into both T.cruzl and Leishmania cells and conferred G418 resistance. Transformation is mediated by large extrachromosomal circular elements composed of head-to-tail tandem repeats of the vector. The transformed phenotype is stable for at least 6 months in the absence of G418 and can be maintained during passage through the T.cruzl ifecycle. Foreign genes Inserted into an expression site within the vector (pTEX) can be expressed at high levels In transformed cells. To our knowledge this paper describes the first trypanosome shuttle vector and the first vector which functions in both trypanosomes and Leishmania.
dc.description.statementofresponsibilityJohn M. Kelly, Helena M. Ward, Michael A. Miles and Giles Kendall
dc.identifier.citationNucleic Acids Research, 1992; 20(15):3963-3969
dc.identifier.doi10.1093/nar/20.15.3963
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.orcidWard, H. [0000-0002-3831-1205]
dc.identifier.urihttp://hdl.handle.net/2440/81798
dc.language.isoen
dc.publisherOxford University Press
dc.rights© 1992 Oxford University Press
dc.source.urihttps://doi.org/10.1093/nar/20.15.3963
dc.subjectAnimals
dc.subjectLeishmania donovani
dc.subjectLeishmania mexicana
dc.subjectTrypanosoma cruzi
dc.subjectGlyceraldehyde-3-Phosphate Dehydrogenases
dc.subjectPhosphotransferases
dc.subjectKanamycin Kinase
dc.subjectRecombinant Fusion Proteins
dc.subjectBlotting, Southern
dc.subjectCloning, Molecular
dc.subjectTransfection
dc.subjectGene Expression
dc.subjectBase Sequence
dc.subjectGenetic Vectors
dc.subjectPlasmids
dc.subjectMolecular Sequence Data
dc.titleA shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania
dc.typeJournal article
pubs.publication-statusPublished

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