Developmental penalties associated with inducible tolerance in Helicoverpa armigera to insecticidal toxins from bacillus thuringiensis

dc.contributor.authorRahman, M.
dc.contributor.authorGlatz, R.
dc.contributor.authorRoush, R.
dc.contributor.authorSchmidt, O.
dc.date.issued2011
dc.description.abstractExposure of insect larvae to sublethal concentrations of crystal toxins from the soil bacterium Bacillus thuringiensis (Bt toxins) causes the induction of immune and metabolic responses that can be transmitted to offspring by epigenetic inheritance mechanisms. Given that the elevated immune status carries significant developmental penalties, we wanted to establish the relationships between immune induction, tolerance to the toxin and developmental penalties. A laboratory culture of Helicoverpa armigera was induced by a sublethal bacterial suspension containing crystal toxin Cry1Ac in one generation and maintained in the presence of toxin, acquiring significant levels of tolerance to the toxin within 12 generations of continuous exposure. Comparing tolerant and susceptible insects, we show that the induction of larval immune response and the coincident alteration of development-related metabolic activities by elicitors in the larval gut (larval induction) differs from the elevated immune status transmitted by epigenetic mechanisms (embryonic induction). Because the damaging effects of larval induction processes are higher compared to embryonic induction, it is likely that overall developmental penalties depend on the relative contribution of the two induction processes. When insects are kept with the same amount of toxin in the diet for subsequent generations, the embryonic induction process increases its contribution compared to the larval induction, resulting in reduced overall developmental penalty, while tolerance to the toxin is maintained.
dc.description.statementofresponsibilityMahbub Rahman, Richard Glatz, Rick Roush, and Otto Schmidt
dc.identifier.citationApplied and Environmental Microbiology, 2011; 77(4):1443-1448
dc.identifier.doi10.1128/AEM.01467-10
dc.identifier.issn0099-2240
dc.identifier.issn1098-5336
dc.identifier.orcidGlatz, R. [0000-0001-8889-3701]
dc.identifier.urihttp://hdl.handle.net/2440/66932
dc.language.isoen
dc.publisherAmer Soc Microbiology
dc.rightsCopyright © 2011, American Society for Microbiology. All Rights Reserved.
dc.source.urihttps://doi.org/10.1128/aem.01467-10
dc.subjectAnimals
dc.subjectMoths
dc.subjectBacillus thuringiensis
dc.subjectBody Weight
dc.subjectBacterial Proteins
dc.subjectBacterial Toxins
dc.subjectEndotoxins
dc.subjectEmbryonic Induction
dc.subjectEpigenesis, Genetic
dc.subjectLarva
dc.subjectInsecticide Resistance
dc.subjectDrug Tolerance
dc.subjectHemolysin Proteins
dc.subjectBacillus thuringiensis Toxins
dc.titleDevelopmental penalties associated with inducible tolerance in Helicoverpa armigera to insecticidal toxins from bacillus thuringiensis
dc.typeJournal article
pubs.publication-statusPublished

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