Periconception onset diabetes is associated with embryopathy and fetal growth retardation, reproductive tract hyperglycosylation and impaired immune adaptation to pregnancy

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dc.contributor.authorBrown, H.
dc.contributor.authorGreen, E.
dc.contributor.authorTan, T.
dc.contributor.authorGonzalez, M.
dc.contributor.authorRumbold, A.
dc.contributor.authorHull, M.
dc.contributor.authorNorman, R.
dc.contributor.authorPacker, N.
dc.contributor.authorRobertson, S.
dc.contributor.authorThompson, J.
dc.date.issued2018
dc.description.abstractDiabetes has been linked with impaired fertility but the underlying mechanisms are not well defined. Here we use a streptozotocin-induced diabetes mouse model to investigate the cellular and biochemical changes in conceptus and maternal tissues that accompany hyperglycaemia. We report that streptozotocin treatment before conception induces profound intra-cellular protein β-O-glycosylation (O-GlcNAc) in the oviduct and uterine epithelium, prominent in early pregnancy. Diabetic mice have impaired blastocyst development and reduced embryo implantation rates, and delayed mid-gestation growth and development. Peri-conception changes are accompanied by increased expression of pro-inflammatory cytokine Trail, and a trend towards increased Il1a, Tnf and Ifng in the uterus, and changes in local T-cell dynamics that skew the adaptive immune response to pregnancy, resulting in 60% fewer anti-inflammatory regulatory T-cells within the uterus-draining lymph nodes. Activation of the heat shock chaperones, a mechanism for stress deflection, was evident in the reproductive tract. Additionally, we show that the embryo exhibits elevated hyper-O-GlcNAcylation of both cytoplasmic and nuclear proteins, associated with activation of DNA damage (ɣH2AX) pathways. These results advance understanding of the impact of peri-conception diabetes, and provide a foundation for designing interventions to support healthy conception without propagation of disease legacy to offspring.
dc.description.statementofresponsibilityHannah M. Brown, Ella S. Green, Tiffany C.Y. Tan, Macarena B. Gonzalez, Alice R. Rumbold, M. Louise Hull, Robert J. Norman, Nicolle H. Packer, Sarah A. Robertson, Jeremy G. Thompson
dc.identifier.citationScientific Reports, 2018; 8(1):2114-1-2114-13
dc.identifier.doi10.1038/s41598-018-19263-8
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidBrown, H. [0000-0001-6342-3316]
dc.identifier.orcidGreen, E. [0000-0002-3009-3442]
dc.identifier.orcidRumbold, A. [0000-0002-4453-9425]
dc.identifier.orcidHull, M. [0000-0003-1813-3971] [0000-0003-4660-4005]
dc.identifier.orcidNorman, R. [0000-0002-3118-3896]
dc.identifier.orcidRobertson, S. [0000-0002-9967-0084]
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]
dc.identifier.urihttp://hdl.handle.net/2440/111645
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.grantNHMRC
dc.relation.grantARC
dc.rights© The Author(s) 2018 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s41598-018-19263-8
dc.subjectEmbryology; endocrine reproductive disorders; glycobiology; mechanisms of disease
dc.titlePericonception onset diabetes is associated with embryopathy and fetal growth retardation, reproductive tract hyperglycosylation and impaired immune adaptation to pregnancy
dc.typeJournal article
pubs.publication-statusPublished

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