Phospholipid composition of reconstituted high density lipoproteins influences their ability to inhibit endothelial cell adhesion molecule expression

Simple item page
dc.contributor.authorBaker, P.
dc.contributor.authorRye, K.A.
dc.contributor.authorGamble, J.
dc.contributor.authorVadas, M.
dc.contributor.authorBarter, P.
dc.date.issued2000
dc.descriptionManuscript received 3 September 1999 and in revised form 9 March 2000.
dc.description.abstractThe ability of different phosphatidylcholine (PC) species to inhibit cytokine-induced expression of vascular cell adhesion molecule 1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) was investigated. PC species containing palmitoyl- in the sn -1 position and palmitoyl- (DPPC), arachidonyl- (PAPC), linoleoyl- (PLPC) or oleoyl- (POPC) in the sn -2 position were compared. These PC species were studied as components of reconstituted high density lipoproteins (rHDL) (containing apolipoprotein A-I [apoA-I] as the sole protein) or as small unilamellar vesicles (SUVs). The rHDL containing PLPC and PAPC inhibited VCAM-1 expression in activated HUVECs by 95 and 70%, respectively, at an apoA-I concentration of 16 m M . At this concentration of apoA-I, POPC rHDL inhibited by only 16% and DPPC rHDL did not inhibit at all. These differences could not be explained by differential binding of the rHDL to HUVECs. The same hierarchy of inhibitory activity was observed when these PC species were presented to the cells as SUVs but only when the SUVs also contained an antioxidant. It was concluded that rHDL PC is responsible for their inhibitory activity and that this varies widely with different PC species. —Baker, P. W., K-A. Rye, J. R. Gable, M. A. Vadas, and P. J. Barter. Phospholipid composition of reconstituted high density lipoproteins influences their ability to inhibit endothelial cell adhesion molecule expression. J. Lipid Res. 2000. 41: 1261–1267.
dc.description.statementofresponsibilityPaul W. Baker, Kerry-Anne Rye, Jennifer R. Gamble, Mathew A. Vadas, and Philip J. Barter
dc.identifier.citationJournal of Lipid Research, 2000; 41(8):1261-1267
dc.identifier.doi10.1016/S0022-2275(20)33434-9
dc.identifier.issn0022-2275
dc.identifier.issn1539-7262
dc.identifier.urihttp://hdl.handle.net/2440/9620
dc.language.isoen
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.rights© 2000 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This is an Open Access article under the CC BY license.
dc.source.urihttps://doi.org/10.1016/s0022-2275(20)33434-9
dc.subjectvascular cell adhesion molecule 1
dc.subjectVCAM-1
dc.subjecthuman umbilical vein endothelial cells
dc.subjectHUVEC TNF-α
dc.subjectIL-1
dc.subjectfatty acid composition
dc.subjectatherosclerosis
dc.titlePhospholipid composition of reconstituted high density lipoproteins influences their ability to inhibit endothelial cell adhesion molecule expression
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_9620.pdf
Size:
382.84 KB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Medicine publications