Comparison of ELISA and RT-PCR for the detection of PNRSV and PDV in Australian almond trees

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dc.contributor.authorMekuria, G.
dc.contributor.authorRamesh, S.
dc.contributor.authorAlberts, E.
dc.contributor.authorBertozzi, T.
dc.contributor.authorWirthensohn, M.
dc.contributor.authorCollins, G.
dc.contributor.authorSedgley, M.
dc.contributor.conferenceGREMPA Meeting on Almonds and Pistachios (13th : 2003 : Mirandela, Portugal)
dc.contributor.editorOliveira, M.
dc.contributor.editorCordeiro, V.
dc.date.issued2003
dc.description.abstractThis three-year study compared the use of ELISA and RT-PCR for identifying Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) on 175 leaf samples taken from trees maintained as a budwood repository for almond growers. The primers used for RT-PCR were based on DNA sequences that code for coat protein, and both viruses could be detected in the same reaction. For PNRSV, both ELISA and RT-PCR produced similar results, although RT-PCR was more consistent, and had the added advantage that plant material could be tested at any time throughout the growing season. For PDV, virus particles were not detected by ELISA, but were detected in low titre using a nested PCR technique. RT-PCR is used routinely now to index progeny developed each year by the Australian almond improvement program in place of both graft incompatibility using woody indicator species and ELISA.
dc.description.statementofresponsibilityG. Mekuria, S.A. Ramesh, E. Alberts, T. Bertozzi, M. Wirthensohn, G. Collins and M. Sedgley
dc.description.urihttp://om.ciheam.org/article.php?IDPDF=5600030
dc.identifier.citationProceedings of the XIIIth Meeting of the Mediterranean Research Group for Almond and Pistachios (GREMPA), 1-5 June, 2003 / M. M. Oliveira, V. Cordeiro (eds.): pp.193-196
dc.identifier.isbn2853523071
dc.identifier.orcidRamesh, S. [0000-0003-2230-4737]
dc.identifier.orcidBertozzi, T. [0000-0001-6665-3395]
dc.identifier.orcidWirthensohn, M. [0000-0003-1174-6580]
dc.identifier.urihttp://hdl.handle.net/2440/41865
dc.language.isoen
dc.publisherCentre International de Hautes Etudes Agronomiques Méditerranénnes
dc.source.urihttp://ressources.ciheam.org/om/pdf/a63/05600030.pdf
dc.subjectPDV
dc.subjectPNRSV
dc.subjectRT-PCR
dc.subjectELISA
dc.subjectPrunus
dc.subjectAlmond
dc.titleComparison of ELISA and RT-PCR for the detection of PNRSV and PDV in Australian almond trees
dc.typeConference paper
pubs.publication-statusPublished

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