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ProBDNF inhibits collective migration and chemotaxis of rat Schwann cells

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dc.contributor.authorDing, Y.Q.
dc.contributor.authorLi, X.Y.
dc.contributor.authorXia, G.N.
dc.contributor.authorRen, H.Y.
dc.contributor.authorZhou, X.F.
dc.contributor.authorSu, B.Y.
dc.contributor.authorQi, J.G.
dc.date.issued2016
dc.description.abstractSchwann cell migration, including collective migration and chemotaxis, is essential for the formation of coordinate interactions between Schwann cells and axons during peripheral nerve development and regeneration. Moreover, limited migration of Schwann cells imposed a serious obstacle on Schwann cell-astrocytes intermingling and spinal cord repair after Schwann cell transplantation into injured spinal cords. Recent studies have shown that mature brain-derived neurotrophic factor, a member of the neurotrophin family, inhibits Schwann cell migration. The precursor form of brain-derived neurotrophic factor, proBDNF, was expressed in the developing or degenerating peripheral nerves and the injured spinal cords. Since "the yin and yang of neurotrophin action" has been established as a common sense, proBDNF would be expected to promote Schwann cell migration. However, we found, in the present study, that exogenous proBDNF also inhibited in vitro collective migration and chemotaxis of RSC 96 cells, a spontaneously immortalized rat Schwann cell line. Moreover, proBDNF suppressed adhesion and spreading of those cells. At molecular level, proBDNF inhibits F-actin polymerization and focal adhesion dynamics in cultured RSC 96 cells. Therefore, our results suggested a special case against the classical opinion of "the yin and yang of neurotrophin action" and implied that proBDNF might modulate peripheral nerve development or regeneration and spinal cord repair through perturbing native or transplanted Schwann cell migration.
dc.identifier.citationTissue and Cell, 2016; 48(5):503-510
dc.identifier.doi10.1016/j.tice.2016.07.002
dc.identifier.issn0040-8166
dc.identifier.issn1532-3072
dc.identifier.orcidZhou, X.F. [0000-0002-8687-0175]
dc.identifier.urihttps://hdl.handle.net/11541.2/122223
dc.language.isoen
dc.publisherCHURCHILL LIVINGSTONE
dc.relation.fundingSichuan Province Science and Technology Support Program 2013SZ0069
dc.relation.fundingSichuan Province Applied Basic Research Program 2010JY0057
dc.relation.fundingSichuan Province Development and Regeneration Key Laboratory Program SYS13-001
dc.relation.fundingScientific Research Foundation for the Young Scholars, Sichuan University 2014SCU11041
dc.rightsCopyright 2016 Elsevier Ltd
dc.source.urihttp://dx.doi.org/10.1016/j.tice.2016.07.002
dc.subjectSpinal Cord
dc.subjectSchwann Cells
dc.subjectPeripheral Nerves
dc.subjectAnimals
dc.subjectRats
dc.subjectSpinal Cord Injuries
dc.subjectBrain-Derived Neurotrophic Factor
dc.subjectNerve Regeneration
dc.subjectCell Movement
dc.subjectChemotaxis
dc.titleProBDNF inhibits collective migration and chemotaxis of rat Schwann cells
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916097384401831

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